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Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

18.6K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Related Experiment Video

Updated: Jan 6, 2026

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
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Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

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Uncovering essential lncRNAs through transcriptome-scale CRISPR-Cas13 screening.

Jiahua Si1, Xinming Su1, Zhuoyan Jin1

  • 1Department of Clinical Medicine, School of Medicine, Hangzhou City University, Hangzhou, Zhejiang, China.

Advanced Biotechnology
|September 15, 2025
PubMed
Summary

Researchers developed CaRPool-seq, a CRISPR-Cas13 screening tool, to identify essential long non-coding RNAs (lncRNAs). This method discovered 778 vital lncRNAs, advancing understanding of gene regulation and disease.

Area of Science:

  • Genomics
  • Molecular Biology
  • RNA Biology
Keywords:
CRISPRCas13LncRNARNA targetingTranscriptome

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Background:

  • The human genome extensively produces non-coding RNAs (ncRNAs), particularly long non-coding RNAs (lncRNAs), which regulate crucial cellular functions.
  • Functional characterization of lncRNAs is challenging due to low sequence conservation, limited abundance, and structural complexity.
  • Existing methods like RNA interference and CRISPR-Cas9 have limitations in efficiency, specificity, and scalability for lncRNA studies.