Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

3.5K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
3.5K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

3.2K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.2K
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

6.2K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
6.2K
SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

12.4K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
12.4K
Nuclear Export01:42

Nuclear Export

4.8K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
4.8K
The Early Endosome: Endocytosis of Transferrin01:28

The Early Endosome: Endocytosis of Transferrin

4.7K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stable overexpression of the epithelial sodium channel alpha subunit reduces migration and proliferation in breast cancer cells.

Breast cancer research and treatment·2025
Same author

Admission albumin-globulin ratio associated with delayed cerebral ischemia following aneurysmal subarachnoid hemorrhage.

Frontiers in neurology·2024
Same author

Computational discovery of two-dimensional tetragonal group IV-V monolayers.

RSC advances·2024
Same author

Nickel-Catalyzed Direct Fluorosulfonylation of Vinyl Bromides and Benzyl Bromides for Sulfonyl Fluorides.

Organic letters·2024
Same author

Preoperative Prediction of Occult Level V Lymph Node Metastasis in Papillary Thyroid Carcinoma: Development and Validation of a Radiomics-Driven Nomogram Model.

Academic radiology·2024
Same author

Self-guided Knowledge-Injected Graph Neural Network for Alzheimer's Diseases.

Medical image computing and computer-assisted intervention : MICCAI ... International Conference on Medical Image Computing and Computer-Assisted Intervention·2024

Related Experiment Video

Updated: Jan 17, 2026

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

4.0K

Sorting nexins associated with recycling complexes regulate epithelial sodium channel trafficking.

Monique L Scott1, Tanya T Cheung1, Matthew J E Logue1

  • 1Department of Physiology, Faculty of Biomedical Sciences, University of Otago, Dunedin, New Zealand.

American Journal of Physiology. Renal Physiology
|September 15, 2025
PubMed
Summary

Sorting nexins (SNX) are crucial for recycling the epithelial sodium channel (ENaC) to the cell surface. This study reveals SNX proteins regulate ENaC levels, impacting kidney salt and water balance.

Keywords:
ENaCSNXretrieverretromertrafficking

More Related Videos

Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers
10:28

Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

Published on: December 6, 2019

8.2K
Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane
07:38

Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane

Published on: March 30, 2015

9.7K

Related Experiment Videos

Last Updated: Jan 17, 2026

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

4.0K
Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers
10:28

Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

Published on: December 6, 2019

8.2K
Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane
07:38

Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane

Published on: March 30, 2015

9.7K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Physiology

Background:

  • The epithelial sodium channel (ENaC) is vital for kidney salt and water homeostasis.
  • Dysregulation of ENaC cell surface levels causes hypertension or hypotension.
  • Protein trafficking complexes, retromer and retriever, control membrane protein recycling via sorting nexins (SNX).

Purpose of the Study:

  • To investigate the role of SNX proteins in the recycling of ENaC.
  • To determine if SNX proteins mediate ENaC interaction with retromer and retriever complexes.

Main Methods:

  • Used epithelial cell lines (FRT, mCCDcl1, HEK293) and transfected human ENaC.
  • Employed siRNA knockdown of SNX proteins (SNX1, 2, 3, 5, 17).
  • Assessed ENaC function via amiloride-sensitive short-circuit current and cell surface levels using biotinylation and coimmunoprecipitation.

Main Results:

  • siRNA knockdown of SNX proteins reduced ENaC current and cell surface population.
  • Coimmunoprecipitation showed ENaC interacts with SNX3 and SNX17.
  • SNX3 and SNX17 likely act as cargo adaptors for ENaC to retromer and retriever complexes.

Conclusions:

  • SNX proteins associated with retromer and retriever complexes are essential for maintaining ENaC cell surface levels.
  • These findings offer new insights into the regulation of ENaC activity and kidney function.
  • SNX proteins play a critical role in controlling ENaC trafficking and physiological ENaC activity.