Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.3K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.9K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.9K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

8.1K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.1K
Nucleic Acid Structure01:25

Nucleic Acid Structure

8.4K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
8.4K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

14.7K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
14.7K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

4.2K
4.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcription Start Site Choice Regulates m<sup>6</sup>A Stoichiometry in Cap-Proximal Regions.

Genes·2026
Same author

Validation of the mRNA epitranscriptome: SCARPET reveals that mapped m<sup>1</sup>A sites are inosine.

EMBO reports·2026
Same author

Impact of zervimesine on the neuroinflammatory biomarker GFAP and related proteomic molecular correlates in plasma of participants from a phase 2 clinical trial in Alzheimer's disease.

Alzheimer's research & therapy·2026
Same author

Understanding the Financial Implications of Antimicrobial Resistance Surveillance in Nepal: Context-Specific Evidence for Policy and Sustainable Financing Strategies.

Antibiotics (Basel, Switzerland)·2026
Same author

Drug Development.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

N<sup>6</sup>-Methyladenosine: an RNA modification as a central regulator of cancer.

Nature reviews. Cancer·2025

Related Experiment Video

Updated: Jan 17, 2026

Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

7.6K

Why does the m6A writer complex require so many proteins?

Kiran Pandey1, Hui Xian Poh1, Samie R Jaffrey1

  • 1Department of Pharmacology, Weill Cornell Medicine, Cornell University, New York, New York, United States of America.

Plos Biology
|September 15, 2025
PubMed
Summary
This summary is machine-generated.

The N6-methyladenosine (m6A) writer complex, crucial for gene expression, uses multiple proteins. Understanding these proteins

More Related Videos

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

7.2K
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.3K

Related Experiment Videos

Last Updated: Jan 17, 2026

Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

7.6K
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

7.2K
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.3K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • RNA Biology

Background:

  • N6-methyladenosine (m6A) is the most prevalent internal modification of eukaryotic messenger RNA (mRNA).
  • m6A plays a critical role in post-transcriptional gene expression regulation.
  • Dysregulation of m6A is implicated in various diseases, notably cancer.

Purpose of the Study:

  • To investigate the composition and function of the m6A writer complex.
  • To explore the necessity of multiple protein subunits within the m6A writer complex.
  • To understand the regulatory mechanisms of m6A and its implications for disease.

Main Methods:

  • This study is a review and exploration of existing research on the m6A writer complex.
  • It synthesizes current knowledge on the protein components and their proposed functions.
  • The discussion focuses on the evolutionary and functional advantages of a multi-subunit complex.

Main Results:

  • The m6A writer complex, responsible for m6A conversion, comprises a single catalytic subunit alongside several non-catalytic proteins.
  • The exact functions of these accessory proteins are not fully elucidated but are hypothesized to be involved in substrate recognition, complex assembly, and regulation.
  • The multi-protein nature of the complex suggests intricate regulatory mechanisms beyond simple catalysis.

Conclusions:

  • The multi-subunit architecture of the m6A writer complex is essential for precise regulation of mRNA methylation.
  • Further research into the individual protein functions within the complex is crucial for understanding m6A's role in health and disease.
  • Elucidating these roles may unveil novel therapeutic targets for m6A-associated pathologies, including cancer.