A sample fluorescent probe based on DNA aptamer mimic of GFP for "turn-on" sensing of ClO

Hypochlorous acid (HClO), a significant reactive oxygen species in biological systems, plays a vital role in both physiological and pathological processes. Irregular HClO concentration changes can cause diseases like inflammation, cardiovascular disorders, and neurodegenerative conditions. Thus, creating a method for fast and sensitive ClO^- detection is essential for biomedicine and bioassays. This study introduces an innovative fluorescent biosensor for ClO^- detection, using a DNA aptamer mimic of green fluorescent protein (DMGFP). By integrating DMGFP with phosphorothioate-modified block DNA, the biosensor achieves a low detection limit of 90.3 nM, with high specificity and a streamlined, cost-effective design. It allows for rapid ClO^- analysis in complex biological samples. The method's efficacy was confirmed in tears, urine, and the RVLM tissue of SIH rats, underscoring its clinical and physiological relevance. Additionally, the biosensor demonstrates significant potential in fluorescence imaging, offering a powerful tool for studying oxidative stress-related conditions and disease diagnosis.