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Draft genome sequence and intact prophage characterisation of multidrug-resistant Acinetobacter baumannii isolate
Montri Yasawong1,2, Parweenuch Santaweesuk1, Manassanan Phatcharaharikarn1
1Program on Environmental Toxicology, Chulabhorn Graduate Institute, Bangkok 10210, Thailand.
Abstract:
Multidrug-resistant Acinetobacter baumannii ABU3 was isolated from a tracheal suction specimen from a 76-year-old female patient in Thailand. The draft genome was sequenced using Illumina NextSeq 550. The genome comprised 95 contigs totaling 3826,273 base pairs with an N50 of 112,717 bp and GC content of 38.89. Digital DNA-DNA hybridisation with A. baumannii ATCC 19606T revealed 80.3 % similarity. MLST assigned the isolate to ST-164, whereas MLST of the core genome showed 99.0 % similarity to cgST-4302. Resistance gene analysis identified bla OXA-23, bla CARB variants, bla ADC-25, and tet(39). Antimicrobial susceptibility testing showed resistance to carbapenems, fluoroquinolones, beta-lactam combinations, extended-spectrum cephalosporins, and penicillins, with sensitivity to aminoglycosides and folate pathway inhibitors. Two intact prophage regions (23.5 and 26.3 kb) were identified containing structural and functional components. Data are available at NCBI under BioProject PRJNA550309.
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