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[Formation of a depside-caffeine complex during cold conservation of coffee leaf samples in a hydro-ethanolic medium.
Comptes Rendus Hebdomadaires Des Seances De L'Academie Des Sciences. Serie D: Sciences Naturelles
|July 18, 1977
Abstract:
The depsides present in coffee leaves are not apparent upon extraction with ethanol if the plant material has been fixed in boiling ethanol then maintained at - 25 degrees C. A complex formed with cafein prevents the extraction and this artefact from cold conservation concerns chlorogenic acid and its isomers. A treatment with chloroform can break the complex and allows the depsides to be dosed. The complex does not seem to exist in living tissues.