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Updated: Jan 17, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

NIR-Activatable, Sequence-Specific Metal-Nucleic Acid Scaffolds for Responsive Uncaging.

Arpit Sharma¹, Man Kshetri¹, Deepak Karna¹

¹Department of Chemistry and Biochemistry, Kent State University, Kent, Ohio, 44242, USA.

Angewandte Chemie (International Ed. in English)

|September 17, 2025

Summary

This summary is machine-generated.

We developed sequence-responsive diagnostic uncaging, a novel method using near-infrared light to activate molecules only when specific DNA or miRNA sequences are present. This advances targeted therapies and diagnostics.

Keywords:

DNA‐mediated electron transfer Molecular machines Near infrared Platinum(IV) complexes

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Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

Area of Science:

Biochemistry
Molecular Biology
Nanotechnology

Background:

Precise molecular activation with analyte specificity and spatiotemporal control is challenging for diagnostics and therapies.
Traditional photo-uncaging lacks tissue penetration and specificity, while analyte-responsive platforms lack external control.

Purpose of the Study:

To introduce sequence-responsive diagnostic uncaging, integrating nucleic acid recognition with near-infrared (NIR)-triggered activation.
To develop a versatile photochemical tool for precision medicine and biosensing.

Main Methods:

Constructed a Pt(IV)-DNA molecular scaffold using click chemistry, incorporating a Pt(IV)-caged reporter, nucleic acid recognition domain, and NIR antenna (IR800).
Utilized DNA-mediated electron transfer (DNA-MET) for photoreduction of Pt(IV) centers upon target hybridization.
Demonstrated uncaging of fluorescent reporters (MCA and BDP) upon NIR irradiation in solution and live cells.

Main Results:

Achieved sequence-responsive uncaging triggered by complementary DNA or miRNA binding.
Demonstrated efficient reporter release and high sequence specificity via DNA-MET.
Validated the platform's functionality in both in vitro and live-cell environments.

Conclusions:

The Pt(IV)-DNA scaffold enables responsive molecular activation with high sequence specificity and spatiotemporal control.
This platform bridges diagnostics and molecular activation, offering potential for precision medicine, targeted drug delivery, and advanced biosensing.