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Updated: Jan 17, 2026
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
The Relationship Between Lipid Peroxidation, Serum Antioxidant Vitamin, Inflammatory Biomarker, Trace Elements, and
Md Ashiqul Islam1,2, Md Ashraful Alam2,3, Shahriar Ahmed2
1Department of Pharmacy Mawlana Bhashani Science and Technology University Tangail Bangladesh.
Background And Aims:
Fibromyalgia is an emerging health concern in Bangladesh, with symptoms that tend to worsen over time. This study sought to measure serum lipid peroxidation, nonenzymatic antioxidant, C-reactive protein (CRP), macro-minerals, and trace elements in fibromyalgia syndrome (FMS) patients to explore their association with disease progression.
Methods:
The investigation involved 50 fibromyalgia patients (cases) and 50 healthy individuals (controls) in Bangladesh. Serum malondialdehyde (MDA) was measured to assess lipid peroxidation utilizing spectrophotometry. UV spectrophotometric technique was employed for the determination of vitamin C, while serum levels of macro-minerals (Mg and Ca) and trace elements (Zn and Cu) were quantified using atomic absorption spectroscopy. CRP level was ascertained by using CRP-Latex agglutination assay.
Results:
Our study identified markedly increased levels of MDA (4.26 ± 0.04 nmol/mL, p < 0.001), CRP (17.13 ± 8.95 mg/L, p < 0.001), and notably reduced vitamin C levels (17.6 ± 1.84 µmol/L, p < 0.001) in the FMS group compared with healthy controls (HC). Mean serum values of Ca, Mg, Zn, and Cu were 7725.2 ± 202.43 µg/dL, 1173.6 ± 6.00 µg/dL, 69.62 ± 0.69 µg/dL, and 144.66 ± 0.80 µg/dL for the patient group and 8718 ± 169.95 µg/dL, 1619.4 ± 10.32 µg/dL, 91.4 ± 0.86 µg/dL, and 115.28 ± 0.96 µg/dL for the control group, respectively. A significant difference was observed for all macro-minerals and trace elements between the FMS and the HC groups. Moreover, Pearson's correlation study revealed a significant positive and negative correlation between Mg and Cu in the FMS group (r = 0.328, p = 0.02) and in the HC group (r = -0.305, p = 0.032), respectively.
Conclusion:
The findings suggest that elevated serum MDA, depleted serum concentrations of vitamin C, and altered trace elements and macro-mineral concentrations characterize the FMS patients in Bangladesh.
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