Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

Applications of IR Spectroscopy: Overview

Applications of IR Spectroscopy: Overview

The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...

Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...

Applications Of NMR In Biology

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Photoacoustic Imaging for Women's Gynecological Health: Advances and Clinical Prospects.

Bioengineering (Basel, Switzerland)·2026

Same author

Noninvasive Detection and Thermal Ablation Therapy of Endometriosis Using Silica-Coated Gold Nanorods.

Advanced nanobiomed research·2026

Same author

Integrative transcriptomics and metabolomics reveal distinct metabolic reprogramming in luminal and triple-negative breast cancer cells.

Molecular omics·2026

Same author

Phenylpropanoid-specific glycosyltransferases from mango and their potential role in defense.

Plant physiology and biochemistry : PPB·2026

Same author

Nanoceria as Next-Generation Immunotherapeutics: Applications in Chronic Inflammation, Cancer, and Tissue Repair.

Journal of nanotheranostics·2026

Same author

Repurposed ciprofloxacin derivatives as potent autophagic-type anticancer agents.

Bioorganic & medicinal chemistry letters·2025

Search research articles

Related Experiment Video

Updated: Jan 17, 2026

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

Advancements in SERS: Revolutionizing Biomedical Analysis and Applications.

Panangattukara Prabhakaran Praveen Kumar¹, Shivanjali Saxena^2,3, Rakesh Joshi³

¹Department of Biomedical Engineering, Institute for Quantitative Health Science and Engineering, Michigan State University, East Lansing, MI 48824, USA.

Nanotheranostics

|September 17, 2025

Summary

Surface-enhanced Raman scattering (SERS) offers ultrasensitive bioanalysis. Advances in nano- and microfabricated SERS sensors, coupled with AI, enhance molecular detection for diagnostics and personalized medicine.

Keywords:

Raman spectroscopy, microfabrication bioanalytical applications, machine learning.bottom-up method top-down method

More Related Videos

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

Related Experiment Videos

Last Updated: Jan 17, 2026

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

Area of Science:

Nanotechnology and Spectroscopy
Biomedical Engineering
Analytical Chemistry

Background:

Surface-enhanced Raman scattering (SERS) provides ultrasensitive molecular detection for bioanalysis.
Analyte signal intensity in SERS depends on plasmonic material surface roughness and nanogap architecture.
Optimized nanogaps (0.5–1.0 nm) between nanoparticles and analytes enable single-molecule detection.

Purpose of the Study:

To review innovations in nano- and microfabricated SERS sensors for biomolecular detection.
To highlight design, fabrication, and functionalization strategies for SERS bioanalysis.
To explore the role of artificial intelligence (AI) in SERS-based diagnostics.

Main Methods:

Review of recent advancements in SERS sensor design and fabrication.
Analysis of functionalization strategies for enhanced biomolecular detection.
Examination of AI applications in SERS spectral processing and pattern recognition.

Main Results:

Nano- and microfabrication techniques have significantly improved SERS substrate efficiency for biomedical applications.
Optimized nanogap engineering is crucial for achieving high Raman enhancement and single-molecule sensitivity.
AI integration shows promise for advanced spectral analysis and diagnostic model development.

Conclusions:

Advanced SERS sensors, enabled by nano- and microfabrication, are revolutionizing bioanalysis.
AI is increasingly vital for optimizing SERS data processing and diagnostic capabilities.
The synergy of SERS and AI paves the way for point-of-care diagnostics and personalized medicine.