Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.1K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.1K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

1.4K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
1.4K
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

2.1K
The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
2.1K
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

4.5K
When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
4.5K
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

2.8K
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
2.8K
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

1.2K
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

0N4R Tau aggregates producing morphologically different and structurally similar "on-path" and "off-path" oligomers.

Chemical communications (Cambridge, England)·2026
Same author

Nanoscale morphological and structural analysis of round and donut oligomers formed by C-terminal domain of TDP-43.

Physical chemistry chemical physics : PCCP·2026
Same author

Cross-study validation and guidance for resampling approaches for partial least squares discriminant analysis in spectral machine learning.

Analytica chimica acta·2026
Same author

Single Amino Acid-Based Control of Fibril Supramolecular Chirality.

The journal of physical chemistry letters·2026
Same author

Biophysical assessment of the molecular mechanisms of Tau aggregation and its role in Alzheimer's disease.

Protein science : a publication of the Protein Society·2026
Same author

Formation of S- and Z-twist supramolecular micro-ropes by peptide stereoisomers.

Nature communications·2026

Related Experiment Video

Updated: Jan 17, 2026

Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds
09:11

Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds

Published on: October 12, 2018

18.9K

Raman spectroscopy for agricultural applications.

Isaac D Juárez1,2, Dmitry Kurouski1,2

  • 1Department of Biochemistry and Biophysics, Texas A&M University, College, Station, TX, United States.

Frontiers in Plant Science
|September 17, 2025
PubMed
Summary
This summary is machine-generated.

This study provides a guide for plant biologists on Raman spectroscopy analysis. It details methods for spectral data interpretation, chemometrics, and extracting biological insights from plant samples.

Keywords:
2D-COSAnovaPLS-DApeak shiftingresponse characterization

More Related Videos

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

135
Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

7.6K

Related Experiment Videos

Last Updated: Jan 17, 2026

Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds
09:11

Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds

Published on: October 12, 2018

18.9K
A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

135
Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

7.6K

Area of Science:

  • Agricultural Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Raman spectroscopy is increasingly used by plant biologists and agriculturalists.
  • Many users lack extensive experience in Raman data analysis.
  • A need exists for method-focused guidance in this field.

Purpose of the Study:

  • To provide a comprehensive guide on Raman spectroscopic data analysis for agricultural applications.
  • To elucidate the differences and complementarity between peak-based and full spectral analysis.
  • To demonstrate the application of chemometric methods for data classification and biological insight extraction.

Main Methods:

  • Critical discussion of key steps in spectroscopic data analysis.
  • Comparison of peak fitting versus full spectral analysis approaches.
  • Application and validation of chemometric methods, including Partial Least Squares Discriminant Analysis (PLS-DA).
  • Demonstration of 2-D correlation spectroscopy for analyzing plant biological processes.

Main Results:

  • Spectral preprocessing, model parameters, and validation significantly impact supervised method performance in data classification.
  • Both peak-based and full spectral analyses offer complementary information.
  • Peak fitting and 2-D correlation spectroscopy are valuable for extracting biological process information.

Conclusions:

  • This work serves as a practical guide for agricultural scientists using Raman spectroscopy.
  • Understanding spectral analysis methods enhances the interpretation of plant biological processes.
  • Chemometric approaches, when properly applied and validated, are powerful tools for Raman data analysis in agriculture.