C-C chemokine receptor type 5 activation stimulates adipocyte differentiation through ERK-dependent pathway
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View abstract on PubMed
Summary
This summary is machine-generated.CCR5 activation by RANTES promotes adipocyte differentiation and obesity through an ERK-dependent pathway. Blocking CCR5 inhibits these effects, highlighting its role in obesity development.
Area Of Science
- Metabolism and Endocrinology
- Immunology and Inflammation
Background
- Obesity is linked to chronic inflammation, with elevated RANTES and CCR5 mRNA in visceral adipose tissue of obese individuals.
- The specific role of CCR5 activation in obesity pathogenesis remains incompletely understood.
Purpose Of The Study
- To investigate the impact of CCR5 activation on adipogenesis.
- To elucidate the regulatory mechanisms underlying CCR5's role in obesity.
Main Methods
- Utilized 3T3-F442A preadipocytes and primary preadipocytes from wild-type (WT) and CCR5 knockout (CCR5-/-) mice.
- Administered RANTES, CCR5 inhibitor (maraviroc), and ERK inhibitor (PD98059) to assess adipocyte differentiation.
- Fed WT and CCR5-/- mice normal chow or high-fat diets to evaluate in vivo effects on obesity.
Main Results
- RANTES treatment increased triglyceride accumulation and adipogenic gene expression (PPARγ, C/EBPα, aP2) in differentiating adipocytes.
- CCR5 inhibition and ERK pathway blockade attenuated RANTES-induced adipocyte differentiation.
- WT mice on high-fat diets exhibited increased RANTES, adipose CCR5 expression, and obesity, unlike CCR5-/- mice.
Conclusions
- CCR5 activation by RANTES enhances adipocyte differentiation via an ERK-dependent mechanism.
- CCR5 plays a significant role in high-fat diet-induced obesity development.
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