Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Search research articles

Home
Development Of A High-performance Liquid Chromatography-fluorescence Method For Determination Of Ethoxyquin And Its Dimer In Antarctic Krill Powder.

Home
Development Of A High-performance Liquid Chromatography-fluorescence Method For Determination Of Ethoxyquin And Its Dimer In Antarctic Krill Powder.

Related Experiment Video

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

Published on: August 1, 2018

Development of a High-Performance Liquid Chromatography-Fluorescence Method for Determination of Ethoxyquin and Its

Lihong Xing^1,2, Weihong Sun^1,2,3, Xiaojie Sun^1,2

¹Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture and Rural Affairs, Qingdao, P. R. China.

Journal of Separation Science

|September 17, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

A new analytical method accurately measures ethoxyquin (EQ) and its metabolite ethoxyquin dimer (EQDM) in Antarctic krill powder. This ensures consumer safety by monitoring preservative levels in krill products.

Keywords:

Antarctic krill powder | ethoxyquin dimer | ethoxyquin | high‐performance liquid chromatography

More Related Videos

Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry

Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry

Published on: March 6, 2016

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

Related Experiment Videos

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

Published on: August 1, 2018

Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry

Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry

Published on: March 6, 2016

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

Area of Science:

Food Chemistry
Analytical Chemistry
Food Safety

Background:

Ethoxyquin (EQ) is a synthetic antioxidant used as a preservative in consumer products and animal feeds.
EQ is commonly added to Antarctic krill products, raising concerns about potential consumer health hazards.
Monitoring EQ levels in krill is crucial for ensuring consumer safety.

Purpose of the Study:

To establish a simultaneous analytical method for determining residual ethoxyquin (EQ) and its primary metabolite, ethoxyquin dimer (EQDM).
To validate the method for application in Antarctic krill powder (AKP) analysis.

Main Methods:

Extraction of residual EQ and EQDM from AKP using sodium carbonate solution, acetone, and n-hexane.
Purification of analytes via neutral alumina solid-phase extraction.

Quantification using high-performance liquid chromatography (HPLC) with fluorescence detection.

Main Results:

The method demonstrated excellent linearity (correlation coefficients >0.999) for both EQ and EQDM.
High recoveries (78.8%–101%) and low relative standard deviations (<15%) indicate accuracy and precision.
Sensitive detection and quantification limits were achieved (LOD: 3 µg/kg for EQ, 30 µg/kg for EQDM; LOQ: 10 µg/kg for EQ, 100 µg/kg for EQDM).

Conclusions:

The developed HPLC method is sensitive, accurate, easy to operate, and economical.
This method is suitable for the routine determination of EQ and EQDM in Antarctic krill powder.
The method supports enhanced quality control and consumer safety for krill-based products.