Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drugs for Treatment of Ulcerative Colitis in IBD01:29

Drugs for Treatment of Ulcerative Colitis in IBD

463
Ulcerative colitis is a chronic inflammatory condition primarily affecting the colon and rectum. The primary drugs used in the treatment of ulcerative colitis are aminosalicylates. They exhibit anti-inflammatory and immunosuppressive properties. They modulate inflammatory mediators and inhibit the activity of nuclear factor κB (NF-κB). Aminosalicylates also reduce inflammation by inhibiting prostaglandin and leukotriene production and decreasing neutrophil chemotaxis and superoxide...
463

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diversity of Iodinated Water Disinfection Byproducts and Their Formation Dynamics Discovered Using Exposome-Scale Nontargeted Analysis and Machine Learning.

Environmental science & technology·2026
Same author

BAGO: A Self-Optimizing Tool for LC-MS Gradient Design in Metabolomics.

Analytical chemistry·2026
Same author

Organoid Level Assessments of Human Primary and Metastatic Colorectal Cancer-Derived Organoids Predict Response to Chemotherapy and Chemoradiation.

Cancers·2026
Same author

The Multifaceted Challenge of Normalizing Mass Spectrometry-Based Metabolomics Data.

Analytical chemistry·2026
Same author

Crystal structure of epidermal growth factor domain-specific <i>O</i>-linked <i>N</i>-acetylglucosamine transferase reveals a conserved N-R-R constellation for uridine diphosphate recognition in the GT61 family.

PNAS nexus·2026
Same author

Disinfection Byproducts in the Long-Tail Exposome: Unmet Challenges for Water Quality and Human Health.

Environmental science & technology·2026

Related Experiment Video

Updated: Jan 17, 2026

Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer
06:24

Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer

Published on: October 13, 2023

1.6K

The Microbial Bile Acid Metabolite 3-Oxo-LCA Inhibits Colorectal Cancer Progression.

Fei Sun1, Ke Wang1, Xingchen Dong1

  • 1Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin.

Cancer Research
|September 17, 2025
PubMed
Summary

The microbial bile acid 3-oxo-lithocholic acid (3-oxo-LCA) activates FXR signaling, inhibiting colorectal cancer stem cell growth and promoting apoptosis. This highlights its potential for treating intestinal tumorigenesis.

More Related Videos

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells
06:07

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells

Published on: June 13, 2020

7.1K
Non-invasive Assessment of the Efficacy of New Therapeutics for Intestinal Pathologies Using Serial Endoscopic Imaging of Live Mice
09:01

Non-invasive Assessment of the Efficacy of New Therapeutics for Intestinal Pathologies Using Serial Endoscopic Imaging of Live Mice

Published on: March 10, 2015

10.4K

Related Experiment Videos

Last Updated: Jan 17, 2026

Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer
06:24

Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer

Published on: October 13, 2023

1.6K
Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells
06:07

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells

Published on: June 13, 2020

7.1K
Non-invasive Assessment of the Efficacy of New Therapeutics for Intestinal Pathologies Using Serial Endoscopic Imaging of Live Mice
09:01

Non-invasive Assessment of the Efficacy of New Therapeutics for Intestinal Pathologies Using Serial Endoscopic Imaging of Live Mice

Published on: March 10, 2015

10.4K

Area of Science:

  • Microbiology
  • Gastroenterology
  • Oncology

Background:

  • Bile acids (BA) are metabolized by gut bacteria into microbial BAs.
  • 3-oxo-lithocholic acid (3-oxo-LCA) and isoallo-LCA modulate immunity, suppress pathogens, and offer antiaging benefits.
  • These microbial BAs may impact intestinal epithelial cells and colorectal cancer (CRC) progression.

Purpose of the Study:

  • To investigate the impact of 3-oxo-LCA on intestinal tumorigenesis.
  • To evaluate 3-oxo-LCA's activity in vitro and in vivo models of colorectal cancer.

Main Methods:

  • In vitro studies utilized mouse and human colorectal cancer cell lines and organoids.
  • In vivo studies employed genetically engineered mouse models, syngeneic and xenograft tumors, and patient-derived xenografts.
  • Assessed FXR signaling activation, cell proliferation, apoptosis, gut barrier function, and tumor burden.

Main Results:

  • 3-oxo-LCA is a potent FXR agonist, restoring FXR signaling in vitro and in vivo.
  • FXR activation by 3-oxo-LCA reduced colorectal cancer cell growth and suppressed intestinal stem cell proliferation.
  • In APCMin/+ mice, 3-oxo-LCA decreased BA levels, enhanced gut barrier function, reduced tumor burden, and suppressed tumor initiation.
  • 3-oxo-LCA inhibited tumor progression in various mouse models and promoted tumor apoptosis.

Conclusions:

  • 3-oxo-LCA functions as an FXR agonist.
  • It inhibits colorectal cancer tumorigenesis and progression by modulating epithelial cell growth and apoptosis.
  • 3-oxo-LCA shows potential for colorectal cancer treatment.