Related Experiment Video
Updated: Jan 17, 2026

17:01
Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS
Published on: July 18, 2013
13.3K
The GravyTrain toolbox for molecular cell biology
1Department of Biomolecular Sciences, The Weizmann Institute of Science, Rehovot, Israel.
Plos One
|September 17, 2025
Summary
GravyTrain offers yeast genetics researchers a versatile toolkit for genomic modifications. This repository enables gene deletions and protein tagging, facilitating complex system studies like yeast autophagy.
Area of Science:
- Molecular Cell Biology
- Yeast Genetics
Background:
- Yeast genetics is a powerful tool for investigating complex molecular cell biology systems.
- Genomic modifications are essential for protein characterization and manipulation.
Purpose of the Study:
- To present GravyTrain, a comprehensive repository of constructs for yeast genomic modifications.
- To showcase the utility of GravyTrain for creating strain libraries for specific research areas, such as yeast autophagy.
Main Methods:
- Developed GravyTrain, a repository featuring a modular cloning scheme for genomic modifications.
- Constructed gene deletion and gene tagging constructs with fusion tags.
- Created a library of yeast strains for studying autophagy.
Main Results:
- GravyTrain provides a modular system for easy shuffling of genetic elements.
- The repository supports robust protein characterization and manipulation through gene tagging.
- A de novo library of yeast strains for autophagy research was successfully constructed.
Conclusions:
- GravyTrain offers a versatile and expandable platform for yeast genomic research.
- The modular design facilitates broader applications of protein tags beyond yeast.
- This repository streamlines the creation of specialized strain collections for complex biological studies.
Related Concept Videos
Applications of Molecular Taxonomy
517
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
517
Molecular Models
43.5K
Physical models representing molecular architectures of chemical compounds play essential roles in understanding chemistry. The use of molecular models makes it easier to visualize the structures and shapes of atoms and molecules.
43.5K
Tissue Homogenization and Cell Lysis
10.0K
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
10.0K
Modern Molecular Taxonomy
599
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
599
Synthetic Biology
5.5K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
Golden rice
Golden rice is a genetically modified...
5.5K
X-ray Diffraction of Biological Samples
4.7K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
4.7K

