Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

479
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
479
Sexually Transmitted Infections01:26

Sexually Transmitted Infections

936
Sexually transmitted infections (STIs) are diseases transmitted primarily through unsafe sexual interactions. Bacteria, viruses, or parasites cause them and can result in severe health complications if untreated.ChlamydiaThe bacterium Chlamydia trachomatis is responsible for the disease Chlamydia, the most common STI in the United States. This peculiar pathogen requires human cells to reproduce, residing intracellularly. The initial infection often goes unnoticed because it typically does not...
936

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Personalized Dosing of Medicines for Children: A Primer on Pediatric Pharmacometrics for Clinicians.

Paediatric drugs·2024
Same author

Antimicrobial Stewardship in Pediatric Emergency Medicine: A Narrative Exploration of Antibiotic Overprescribing, Stewardship Interventions, and Performance Metrics.

Children (Basel, Switzerland)·2024
Same author

Trends in Antibiotic Use in a Large Children's Hospital in London (United Kingdom): 5 Years of Point Prevalence Surveys.

Antibiotics (Basel, Switzerland)·2024
Same author

Pharmacokinetics of Antimicrobials in Children with Emphasis on Challenges Faced by Low and Middle Income Countries, a Clinical Review.

Antibiotics (Basel, Switzerland)·2023
Same author

Hypocalcemia as a Cause of Complex Febrile Seizures in a Toddler.

Case reports in pediatrics·2021
Same author

Macrolides in children: judicious use, avoiding resistance and reducing adverse effects.

Archives of disease in childhood. Education and practice edition·2021

Related Experiment Video

Updated: Jan 17, 2026

Author Spotlight: Advancing Syphilis Research — Innovations in Treponema pallidum Cultivation and Genetic Engineering
09:45

Author Spotlight: Advancing Syphilis Research — Innovations in Treponema pallidum Cultivation and Genetic Engineering

Published on: January 24, 2025

6.9K

How to interpret syphilis testing.

Faisal Ali1, Sabrina Ye2, Kevin Meesters3

  • 1Pediatrics, The University of British Columbia Faculty of Medicine, Vancouver, British Columbia, Canada faisal.ali@phsa.ca.

Archives of Disease in Childhood. Education and Practice Edition
|September 17, 2025
PubMed
Summary

Syphilis diagnosis is challenging due to non-specific symptoms and difficulty culturing the bacteria. This study reviews direct detection and serological tests, including treponemal (TT) and non-treponemal (NTT) tests, for accurate syphilis identification.

Keywords:
adolescent healthcommunicable diseasesinfectious disease medicineneonatologypaediatrics

More Related Videos

Sexual Transmission of American Trypanosomes from Males and Females to Naive Mates
13:55

Sexual Transmission of American Trypanosomes from Males and Females to Naive Mates

Published on: January 27, 2019

15.6K
Author Spotlight: Advancing Immune Monitoring in Critical Care Patients Using Whole Blood Assays
06:03

Author Spotlight: Advancing Immune Monitoring in Critical Care Patients Using Whole Blood Assays

Published on: September 20, 2024

1.7K

Related Experiment Videos

Last Updated: Jan 17, 2026

Author Spotlight: Advancing Syphilis Research — Innovations in Treponema pallidum Cultivation and Genetic Engineering
09:45

Author Spotlight: Advancing Syphilis Research — Innovations in Treponema pallidum Cultivation and Genetic Engineering

Published on: January 24, 2025

6.9K
Sexual Transmission of American Trypanosomes from Males and Females to Naive Mates
13:55

Sexual Transmission of American Trypanosomes from Males and Females to Naive Mates

Published on: January 27, 2019

15.6K
Author Spotlight: Advancing Immune Monitoring in Critical Care Patients Using Whole Blood Assays
06:03

Author Spotlight: Advancing Immune Monitoring in Critical Care Patients Using Whole Blood Assays

Published on: September 20, 2024

1.7K

Area of Science:

  • Infectious Diseases
  • Microbiology
  • Clinical Diagnostics

Background:

  • Rising global rates of syphilis, including congenital syphilis, present a significant public health challenge.
  • Accurate diagnosis of syphilis is complicated by non-specific clinical symptoms and the inability to culture *Treponema pallidum* using standard laboratory methods.

Purpose of the Study:

  • To review and elucidate various diagnostic methods for syphilis, focusing on their application and interpretation in diverse clinical scenarios.
  • To highlight the utility of direct detection techniques and serological assays (treponemal and non-treponemal tests) in diagnosing syphilis, particularly in early stages and in neonates.

Main Methods:

  • Exploration of direct detection techniques for early syphilis diagnosis prior to seroconversion.
  • Analysis of serological testing, including treponemal tests (TT) for detecting specific antibodies and non-treponemal tests (NTT) for monitoring disease activity.
  • Discussion of diagnostic challenges in neonates with suspected congenital syphilis, emphasizing maternal antibody transfer and the need for clinical correlation.

Main Results:

  • Direct detection methods offer early diagnosis before serological markers are evident.
  • Treponemal tests confirm past or present infection, while non-treponemal test titers correlate with active disease.
  • Interpretation of serological results in neonates requires careful consideration of maternal antibodies and clinical presentation.

Conclusions:

  • A combination of diagnostic approaches, including direct detection and carefully interpreted serological tests (TT and NTT), is crucial for accurate syphilis diagnosis.
  • Understanding the limitations of each test, especially in specific populations like neonates, is essential for effective clinical management of syphilis.