Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Volatilization01:10

Volatilization

5.2K
Volatilization gravimetry is an analytical technique that measures the mass lost due to the volatilization of the substance. This technique is used to estimate the amount of volatile material in a sample. To perform this method, heat a known amount of the sample to a high temperature in a crucible or other suitable vessel. The volatile substance in the sample evaporates, and the vapor is completely expelled from the crucible either by heating the sample or bubbling a stream of inert gas through...
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reply.

Gastroenterology·2026
Same author

A novel antiviral strategy targeting human metapneumovirus through pH modulation in human airway epithelial cells.

The Journal of general virology·2026
Same author

Environmental redox conditions and strain variation define phenazine-mediated antagonism in co-infecting bacteria.

PLoS biology·2026
Same author

Time spectrum repaint domain-wise diffusion model for bearing fault diagnosis under time-varying conditions in high-speed trains.

ISA transactions·2026
Same author

Understanding university teachers' continuance of an AI teaching assistant: an integrated TTF-TAM-ECM model in higher education.

Frontiers in psychology·2026
Same author

A novel antiviral strategy targeting human metapneumovirus through pH modulation in human airway epithelial cells.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jan 17, 2026

Breath Collection from Children for Disease Biomarker Discovery
06:09

Breath Collection from Children for Disease Biomarker Discovery

Published on: February 14, 2019

7.3K

Parallel Offline Breath Sampling for Cross-Validated Analysis of Volatile Organic Compound Metabolites.

Eray Schulz1,2, Mariana Maciel2, Zhige Wang3

  • 1Chemistry & Chemical Biology, Indiana University Indianapolis, Indianapolis, IN, USA.

Metabolomics : Official Journal of the Metabolomic Society
|September 17, 2025
PubMed
Summary

Breath volatile organic compounds (VOCs) can indicate health conditions. This study validated two sampling methods, finding consistent results that enhance the reliability of VOC biomarkers for non-invasive health monitoring.

Keywords:
Chemometric analysisExhaled breathGas chromatography–mass spectrometry (GC-MS)Solid phase microextraction (SPME)Volatile organic compounds (VOCs)

More Related Videos

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
08:23

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Published on: March 9, 2018

9.3K
Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
09:19

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

4.5K

Related Experiment Videos

Last Updated: Jan 17, 2026

Breath Collection from Children for Disease Biomarker Discovery
06:09

Breath Collection from Children for Disease Biomarker Discovery

Published on: February 14, 2019

7.3K
Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
08:23

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Published on: March 9, 2018

9.3K
Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
09:19

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

4.5K

Area of Science:

  • Biomarker Discovery
  • Analytical Chemistry
  • Respiratory Medicine

Background:

  • Volatile organic compounds (VOCs) in breath offer potential for non-invasive health monitoring.
  • Challenges in VOC biomarker fidelity exist due to non-standardized sampling methods, risking false discoveries.

Purpose of the Study:

  • To profile VOCs in breath using two offline collection/analysis methods: direct solid phase microextraction (SPME) and Tedlar bag collection.
  • To assess the reliability and reproducibility of identified VOC biomarkers.

Main Methods:

  • 158 participants provided cross-sectional breath samples using SPME and Tedlar bags.
  • 10 participants provided longitudinal samples for reproducibility assessment.
  • Gas chromatography-mass spectrometry (GC-MS) coupled with SPME was employed, alongside robust data processing.

Main Results:

  • Over 30 unique VOCs were identified by both methods.
  • Terpenoids showed consistent trends across both datasets.
  • 11 out of 12 common VOCs showed significant correlations (p < 0.05) in healthy individuals.
  • Longitudinal samples demonstrated higher VOC reproducibility than cross-sectional samples.

Conclusions:

  • Quantitative VOC profiles from both SPME and Tedlar bag methods were congruent.
  • The study enhances the reliability and fidelity of reported breath VOC biomarkers.
  • Biostatistical analysis supported the robust findings of reproducible VOCs.