Related Experiment Video
Updated: Jan 17, 2026
Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
Published on: May 4, 2018
Single high-dose vitamin D supplementation impacts ultramarathon-induced changes in serum levels of bone turnover
Błażej Stankiewicz1, Andrzej Kochanowicz2, Paulina Brzezińska2
1Department of Theory and Methodology of Physical Education and Sport, The Faculty of Health Sciences and Physical Education, Bydgoszcz, Poland.
Background:
Recent studies indicate a protective role of vitamin D supplementation against sports performance-induced dysregulation of body homeostasis. However, the effects of a single high dose of vitamin D on changes in bone formation and resorption markers due to ultramarathon running have yet to be explored. This study aimed to analyze the effect of a single high-dose vitamin D supplementation on serum levels of bone turnover markers after a mountain ultramarathon run.
Methods:
In this clinical trial (reg. number NCT03417700), 35 semiprofessional male ultramarathon runners were assigned into two groups: supplemented group, administered a single high dose of vitamin D3 (cholecalciferol, 150,000 IU) in vegetable oil 24 h before the start of the run (n = 16), and placebo group (n = 19), administered placebo solution 24 h before the start of the run. Blood samples were collected for analysis at three timepoints: 24 h before, immediately after, and 24 h after the run.
Results:
Serum 25(OH)D3 level significantly increased (p ≤ 0.05.) after the ultramarathon in both groups. The increase was more pronounced in the supplemented population, especially 24 h after the run (147.01% vs 84.71%). According to post-hoc and other analyses, the levels of N-terminal propeptides of type I collagen, a PINP marker, were increased immediately after the run. The increase was significantly higher in the supplemented group than in the control group. CTX, PTH, sclerostin, and procalcitonin levels were significantly higher 24 h after the run in the control group.
Conclusions:
The observed attenuation of post-exercise bone resorption and enhancement of bone formation suggest that vitamin D supplementation may modulate bone metabolism in response to extreme physical exertion, potentially through effects on calcium - PTH homeostasis.
More Related Videos
Related Concept Videos
01:23Role of Skin in Vitamin D Synthesis
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin...
01:25Role of Vitamins in Maintaining Bone Health
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
01:11Bioavailability Study Design: Single Versus Multiple Dose Studies
01:21Skeleton and Calcium Homeostasis
01:20Study Designs in Epidemiology
Observational studies are those where the researcher does not intervene but rather observes natural variations. They include cross-sectional, cohort, and...
01:17Hormones and Bone Tissue
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...