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A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation
Published on: December 9, 2021
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Capturing systemic inflammation in a nonhuman primate using a new leukocyte activity assessment system.
Kozo Takeuchi1, Shigeyuki Yamamoto1, Yukiko Hatano1,2
1Central Research Laboratory, Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamana-ku, Hamamatsu, Shizuoka 434-8601, Japan.
Journal of Clinical Biochemistry and Nutrition
|September 18, 2025
Summary
This study introduces a new optical system to monitor leukocyte reactive oxygen species (ROS) in nonhuman primates. The system effectively captures systemic inflammation, correlating ROS production with neutrophil counts and C-reactive protein levels.
Area of Science:
- Biomedical Engineering
- Immunology
- Veterinary Medicine
Background:
- Leukocyte reactive oxygen species (ROS) are crucial in immune responses.
- Monitoring ROS production offers insights into inflammatory conditions.
- Objective monitoring tools are needed for animal health, especially in veterinary medicine.
Purpose of the Study:
- To report the first use of a novel optical system for simultaneous monitoring of leukocyte superoxide and hypochlorite production in nonhuman primates.
- To assess the system's ability to detect systemic inflammation in rhesus monkeys.
- To explore correlations between leukocyte ROS production and inflammatory markers.
Main Methods:
- Development of an optical system for simultaneous detection of leukocyte ROS (superoxide and hypochlorite).
- Application of the system to analyze circulating blood from rhesus monkeys.
- Observational pilot study involving nonhuman primates undergoing unrelated procedures.
Main Results:
- Surgical inflammation was found to influence total leukocyte ROS production over time.
- A septic rhesus monkey showed a distinct pattern of leukocyte ROS production.
- Leukocyte ROS production correlated positively with neutrophil counts at normal C-reactive protein (CRP) levels, with this correlation diminishing under abnormal CRP levels.
Conclusions:
- The developed optical system can effectively capture systemic inflammation in nonhuman primates.
- Monitoring leukocyte ROS production is important for understanding animal inflammatory status.
- Further research with larger cohorts and conventional biomarkers is necessary to fully elucidate the diagnostic potential of leukocyte ROS monitoring in veterinary medicine.
