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MSI.EAGLE: An Open-Source GUI for Streamlined Mass Spectrometry Imaging Analysis
Daniel J Boehmler1,2,3,4, Ashley Woolfork3,4, Ai Wen Tan3,4
1Department of Cancer Biology, University of Pennsylvania, Philadelphia, PA.
None:
Mass Spectrometry Imaging (MSI) is an emergent tool for analyzing spatial molecular distributions, yet data complexity often hinders effective analysis. MSI.EAGLE, an open-source R-Shiny application, makes analysis accessible for non-specialists by integrating advanced tools in a user-friendly interface. The workflow leverages tools from the Cardinal MSI package, with enhanced phenotyping, segmentation, statistical analysis and visualization. The application addresses a gap in spatial biology research by empowering a broader scientific community.
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