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Updated: Jan 17, 2026
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
Published on: August 16, 2016
De novo design of semisynthetic protein nanopores
Lee Schnaider1,2, A Katherine Hatstat1,2, Alistair J Scott3
1Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, USA.
Abstract:
Protein nanopores are essential components of single-molecule oligonucleotide sequencing and sensing devices. Here, we demonstrate that installing additional de novo subunits enables large-scale architectural changes of nanopore complexes. We design de novo proteins that integrate seamlessly with the CsgG pore to form 18-subunit, 315-kilodalton complexes with precisely sculpted pore architectures and tailored ion conduction, opening new possibilities for engineering enhanced nanopores with customized structural and functional properties.
