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Published on: June 3, 2018
Absence of Glaucoma in Tg-MYOCY437H Mice of Diverse Genetic Backgrounds
Nicholas G Tolman1,2,3, Marina Simón1,3, Felicia A Juarez1,3
1Department of Ophthalmology, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, New York, United States.
Purpose:
Mutations in the myocilin (MYOC) gene cause elevated intraocular pressure and glaucoma. To better understand the factors influencing susceptibility to glaucoma, we studied the MYOC mutation (MYOCY437H).
Methods:
We characterized ocular phenotypes in Tg-MYOCY437H mice on nine different mouse strain backgrounds.
Results:
No glaucoma-related phenotypes were observed. We detected neither elevated intraocular pressure (daytime readings) nor optic nerve degeneration differences between wild type (WT) and Tg-MYOCY437H mice. This included an absence of Tg-MYOCY437H-induced phenotypes on a mixed B6SJL background that best reflected the original publications with this strain. We confirmed that this result was not due to an absence of transgene expression in ocular tissues.
Conclusions:
Our data indicate undefined complexity and that the previously reported glaucoma phenotypes are not robust across all institutions and environments.
