Understanding phage Receptor-binding protein interaction with host surface receptor: the key for phage-Mediated...

Area of Science:

• Microbiology
• Virology
• Biotechnology

Background:

• Pseudomonas aeruginosa presents a significant clinical challenge due to antimicrobial resistance and biofilm formation.
• Bacteriophages (phages) are viruses that infect bacteria and are being explored as an alternative to combat multidrug-resistant infections.

Purpose of the Study:

• To review the taxonomic diversity of phages infecting P. aeruginosa.
• To synthesize knowledge on phage receptor-binding proteins (RBPs) and their P. aeruginosa receptors.
• To highlight the need for understanding RBP-receptor interactions for optimizing phage therapy and diagnostics.

Main Methods:

• Systematic review of literature on P. aeruginosa phages.
• Analysis of known phage receptor-binding proteins (RBPs) and their bacterial receptors.
• Synthesis of current knowledge on phage-host interactions.

Main Results:

• Identified diverse P. aeruginosa phages, including unclassified genera like Pbunavirus and Pakpunavirus.
• Detailed common RBPs and their P. aeruginosa surface receptors (LPS, pili, flagella, outer membrane proteins, alginate).
• Emphasized the critical need for understanding the molecular mechanisms of RBP-receptor binding.

Conclusions:

• Deciphering RBP-receptor interactions is crucial for enhancing phage therapy efficacy.
• This knowledge can guide the development of engineered phages and RBP-based antimicrobials.
• Understanding these interactions is vital for creating sensitive, phage-derived diagnostic tools for P. aeruginosa detection.