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Xanthohumol exerts protective function on adriamycin-induced podocyte injury
Huihui Chen1,2, Hongzhou Lin1,2, Rengcheng Qian1,2
1Department of Pediatrics, The Second School of Medicine, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
European Journal of Nutrition
|September 18, 2025
Summary
Xanthohumol (XN) protects against nephrotic syndrome by reducing podocyte injury. This natural compound may offer a new therapeutic option for kidney disease patients.
Area of Science:
- Nephrology
- Molecular Biology
- Pharmacology
Background:
- Nephrotic syndrome (NS) is a leading cause of end-stage renal disease (ESRD).
- Podocyte injury is a key factor in NS progression and proteinuria.
- Identifying novel therapeutic agents for podocyte protection is crucial.
Purpose of the Study:
- To investigate the protective effects of xanthohumol (XN) against podocyte injury.
- To evaluate XN's potential in mitigating adriamycin-induced nephropathy (AN).
Main Methods:
- Established in vivo and in vitro models of adriamycin (ADR)-induced podocyte injury.
- Utilized biochemical assays, PAS staining, immunohistochemistry, immunofluorescence, western blot, and RT-qPCR to assess XN's effects.
- Analyzed the impact of XN on podocyte-specific markers and injury indicators.
Main Results:
- XN upregulated key podocyte markers: Wilms' tumor protein 1 (Wt1), Nephrin, Synaptopodin, Zonula occludens 1 (ZO-1), and Crumbs2 (Crb2).
- XN downregulated the podocyte injury marker Desmin.
- XN treatment mitigated podocyte actin cytoskeleton disruption in ADR-treated models.
Conclusions:
- Xanthohumol (XN) significantly attenuated adriamycin-induced podocyte injury.
- XN demonstrates potential as a therapeutic strategy for nephrotic syndrome (NS).
- Further research into XN for NS treatment is warranted.
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