Protocol for quantifying amino acids in small volumes of human sweat samples

Sweat, a biofluid rich in water, ions, and amino acids, is emerging as a valuable, non-invasive source for continuous health monitoring and disease diagnostics. Here, we present a protocol for amino acid analysis in small volumes of sweat samples. We describe steps for sweat collection, fluorescence derivatization of amino acids, and liquid chromatography analysis, which can be applied to the analysis of very small volumes of sweat samples. For complete details on the use and execution of this protocol, please refer to Tsunoda et al.¹ and Kuroki et al.².