Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

6.5K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
6.5K
Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

8.7K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Diagnosis and treatment of intracranial and extracranial complications of acute suppurative otitis media in children].

Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery·2026
Same author

NeRF-Based Three-Dimensional Reconstruction for Large-Diameter Rescue Shafts.

Sensors (Basel, Switzerland)·2026
Same author

N-myristoylation-mediated shuttling of TaMP from plasma membrane to chloroplasts increases wheat susceptibility to rust fungi.

Nature plants·2026
Same author

Extracellular vesicle-inspired liposomes boost immune response against pathogen antigens.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

Formation of assembloids by DNA-mediated synthetic cell self-assembly.

Soft matter·2026
Same author

Stress granule phase separation in stress-responsive cytosolic extract-in-oil droplets.

Nature communications·2026

Related Experiment Video

Updated: Jan 17, 2026

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
10:44

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

Published on: February 2, 2011

49.4K

Restoring NK Cell Cytotoxicity Post-Cryopreservation via Synthetic Cells.

Xiangda Zhou1,2, Sijia Zhang1, Wenjuan Yang1

  • 1Department of Biophysics, Center for Integrative Physiology and Molecular Medicine (CIPMM), School of Medicine, Saarland University, 66421, Homburg, Germany.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|September 18, 2025
PubMed
Summary
This summary is machine-generated.

Short co-culture with T cells revitalizes cryopreserved natural killer (NK) cells. This method restores NK cell killing in 3D environments, offering a scalable solution for immunotherapy.

Keywords:
3DIL‐2NK cellsT cellscryopreservationcytotoxicitysynthetic cells

More Related Videos

Natural Killer NK and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line
11:02

Natural Killer NK and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line

Published on: February 8, 2022

6.3K
Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
06:53

Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

Published on: January 13, 2023

3.6K

Related Experiment Videos

Last Updated: Jan 17, 2026

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
10:44

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

Published on: February 2, 2011

49.4K
Natural Killer NK and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line
11:02

Natural Killer NK and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line

Published on: February 8, 2022

6.3K
Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
06:53

Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

Published on: January 13, 2023

3.6K

Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Natural killer (NK) cells are crucial for innate immunity and cancer cell elimination.
  • Cryopreservation of NK cells for immunotherapy impairs their cytotoxic function, especially in 3D settings.
  • Existing methods for NK cell storage limit their therapeutic efficacy.

Purpose of the Study:

  • To investigate methods for restoring NK cell functionality after cryopreservation.
  • To explore the role of T cell co-culture in enhancing NK cell performance.
  • To develop a donor-independent strategy for revitalizing cryopreserved NK cells.

Main Methods:

  • Short-term co-culture of cryopreserved NK cells with effector T cells.
  • Assessment of NK cell motility and killing functionality in 3D environments.
  • Development and testing of synthetic T cells engineered with surface-bound IL-2.

Main Results:

  • One-day co-culture with T cells significantly restored NK cell motility and cytotoxicity impaired by cryopreservation.
  • Enhanced NK cell function in 3D environments was dependent on direct cell-cell contact and localized IL-2.
  • Synthetic T cells with surface-bound IL-2 effectively revitalized cryopreserved NK cells.

Conclusions:

  • Direct contact-mediated IL-2 signaling is critical for rescuing NK cell function post-cryopreservation.
  • This approach offers an efficient, cost-effective, and tunable strategy to improve NK cell-based immunotherapies.
  • The findings pave the way for more scalable and clinically viable NK cell therapies.