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CATION AMINO ACID TRANSPORTER1 encodes an arginine transporter whose expression is influenced by daylength and
Marianna Pasquariello1, Yue Qu2, Adam Gauley1,3
1Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
Annals of Botany
|September 18, 2025
Summary
The Photoperiod-1 (Ppd-1) gene in wheat influences nutrient transport by regulating the CATION AMINO ACID TRANSPORTER1 (CAT1) gene. This impacts flowering, root growth, and spikelet development, revealing a broader role for Ppd-1 in reproductive growth.
Area of Science:
- Plant Biology
- Molecular Genetics
- Agricultural Science
Background:
- Wheat flowering and inflorescence development depend on nutrient remobilization.
- The Photoperiod-1 (Ppd-1) gene is crucial for inflorescence development but its role in nutrient transport gene expression is unknown.
Purpose of the Study:
- To investigate if Ppd-1 influences the expression of nutrient transporter genes.
- To elucidate the role of Ppd-1 in wheat reproductive development and nutrient allocation.
Main Methods:
- Transcriptome analysis of wheat near-isogenic lines with variant Ppd-1 alleles.
- Functional analysis of CATION AMINO ACID TRANSPORTER1 (CAT1) using Xenopus oocytes.
- Characterization of mutant lines lacking functional CAT-D1 and CAT-B1.
Main Results:
- Ppd-1 is essential for CAT1 expression in developing wheat inflorescences and leaves.
- CAT1 activity is photoperiod-responsive, with expression increasing with daylength.
- CAT1 transports cationic amino acids, influencing amino acid levels, root growth, and spikelet development.
Conclusions:
- Ppd-1 plays a broader role in wheat reproductive development than previously known.
- Ppd-1 affects the expression of amino acid transporters like CAT1, impacting source and sink tissue growth.
- CAT1, an arginine transporter, is a key mediator in Ppd-1's influence on wheat development.
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