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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Related Experiment Video

Updated: Jun 29, 2026

Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization LNA flow-FISH: a Method for Bacterial Small RNA Detection
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A NanoLock-enabled, Craspase-based strategy for highly sensitive RNA detection.

Yumeng Xiao1, Junyu Chen1, Xincan Hou1

  • 1State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, China.

Nucleic Acids Research
|September 18, 2025
PubMed
Summary

A new CRISPR-based diagnostic platform offers rapid, amplification-free RNA detection. This technology, called Craspase-NanoLock-Csx30 (CNC), achieves high sensitivity for detecting viral RNA in minutes.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Diagnostic Assays

Background:

  • Current RNA detection methods often require amplification, are time-consuming, and risk contamination.
  • There is a need for rapid, sensitive, and specific RNA detection tools in diagnostics.

Purpose of the Study:

  • To develop an amplification-free assay for rapid and sensitive RNA detection.
  • To introduce the CRISPR-guided caspase (Craspase)-NanoLock-Csx30 (CNC) platform for diagnostics.

Main Methods:

  • Utilized a type III-E CRISPR-CRISPR-associated system integrated with NanoLock technology.
  • Developed the CNC platform combining Craspase protease activity and NanoLock luminescence.
  • Applied the CNC platform for detecting specific viral RNA targets.

Main Results:

  • Achieved a detection limit of 250 fM for SARS-CoV-2 N gene RNA in 10 minutes without amplification.
  • Demonstrated high sensitivity and specificity in RNA detection.
  • Showcased potential for detecting Influenza A virus and HIV RNA.

Conclusions:

  • The CNC platform provides a rapid, sensitive, and amplification-free approach for RNA diagnostics.
  • This technology has significant potential for infectious disease detection.
  • Expands the applications of CRISPR-based diagnostic tools.