Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Synchronized replication of Mycobacterium tuberculosis.

L G Wayne

    Infection and Immunity
    |September 1, 1977
    PubMed
    Summary

    Mycobacterium tuberculosis replication was synchronized by sedimenting bacilli. Ribonucleic acid synthesis began immediately, but deoxyribonucleic acid synthesis was delayed until after the first cell division.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Trends in applied mycobacteriology.

    World journal of microbiology & biotechnology·2014
    Same author

    Comparison of measured ozone in southeastern Virginia with computer predictions from a photochemical model. Comments.

    Environmental science & technology·2012
    Same author

    In Vitro Model of Hypoxically Induced Nonreplicating Persistence of Mycobacterium tuberculosis.

    Methods in molecular medicine·2011
    Same author

    Nonreplicating persistence of mycobacterium tuberculosis.

    Annual review of microbiology·2001
    Same author

    Microaerophilic induction of the alpha-crystallin chaperone protein homologue (hspX) mRNA of Mycobacterium tuberculosis.

    Journal of bacteriology·2001
    Same author

    Nitrate reduction as a marker for hypoxic shiftdown of Mycobacterium tuberculosis.

    Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2000

    Area of Science:

    • Microbiology
    • Cell Biology
    • Molecular Biology

    Background:

    • Mycobacterium tuberculosis (M. tuberculosis) is a bacterial species responsible for tuberculosis.
    • Understanding M. tuberculosis replication dynamics is crucial for developing effective treatments.

    Purpose of the Study:

    • To investigate the replication patterns of M. tuberculosis under specific growth conditions.
    • To analyze the timing of nucleic acid synthesis during synchronous cell division.

    Main Methods:

    • Culturing M. tuberculosis in static Tween-albumin broth.
    • Inducing synchronous replication by resuspending settled bacilli.
    • Monitoring ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) synthesis.

    Main Results:

    • M. tuberculosis replicated in the oxygen-rich upper layer of static broth.
    • Sedimented bacilli, when resuspended, exhibited synchronous replication.
    • RNA synthesis initiated immediately upon resuspension.
    • Significant DNA synthesis was observed only after the first cell division, approximately 14 hours post-resuspension.

    Conclusions:

    • Static culture conditions can be used to synchronize M. tuberculosis replication.
    • There is a temporal separation between the initiation of RNA and DNA synthesis during the initial stages of synchronous division in M. tuberculosis.

    Related Experiment Videos