Antibacterial activity of silver nanoparticles against MDR Corynebacterium pseudotuberculosis isolated from caseous
Eman M Elghazaly1, Hebatallah M Saad2, Samy A Khaliel3
1Department of Microbiology, Faculty of Veterinary Medicine, MatrouhUniversity, MarsaMatruh, 51744, Egypt.
Abstract:
Corynebacterium pseudotuberculosis (C. pseudotuberculosis) is a zoonotic pathogen with a poor treatment outcome. It causes a number of chronic disorders, such as caseous lymphadenitis (CLA) in sheep and goats. Also, it can spread to humans and cause potential health events. 120 samples from infected organs and abscessed lymph nodes of sheep and goats with caseous lymphadenitis pyogranulomas were isolated. 10 % (12) of samples were positive for C. pseudotuberculosis by phenotypic methods, pathogenicity testing of guinea pigs, and PCR for the 16 S rRNA. Then they were characterized for their antibiotic susceptibility. We studied the effect of silver nanoparticles (Ag-NPs) on a pan-drug-resistant strain of C. pseudotuberculosis that showed resistance to six classes of antibiotics. In this study, the minimum inhibitory concentration (MIC) was 0.1 mg/ml (100 ppm) and the minimum bactericidal concentration (MBC) of Ag-NPs were 0.05 mg/ml (50 ppm). In this study, the effects of Ag-NPs on the C. pseudotuberculosis morphology, indicated by scanning electron microscopy (SEM) at Matrouh Governorate, revealed pronounced morphological changes indicating damage and rupture of the bacterial membrane. In conclusion, Ag-NPs could be a promising treatment for caseous lymphadenitis pyogranulomas.
Insights
Silver nanoparticles (Ag-NPs) show promise in treating pan-drug-resistant Corynebacterium pseudotuberculosis, the cause of caseous lymphadenitis in livestock. Ag-NPs effectively damaged the bacteria
Area of Science:
- Veterinary Microbiology
- Nanotechnology
- Infectious Diseases
Background:
- Corynebacterium pseudotuberculosis (C. pseudotuberculosis) is a zoonotic pathogen causing chronic diseases like caseous lymphadenitis (CLA) in sheep and goats.
- This pathogen poses a risk to human health and exhibits poor treatment outcomes due to antibiotic resistance.
- Pan-drug-resistant strains present a significant challenge in veterinary medicine.
Purpose of the Study:
- To investigate the efficacy of silver nanoparticles (Ag-NPs) against a pan-drug-resistant strain of C. pseudotuberculosis.
- To determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Ag-NPs.
- To evaluate the morphological effects of Ag-NPs on C. pseudotuberculosis using scanning electron microscopy (SEM).
Main Methods:
- Isolation and identification of C. pseudotuberculosis from infected sheep and goat samples.
- Phenotypic methods, pathogenicity testing in guinea pigs, and PCR for 16S rRNA gene for confirmation.
- Antibiotic susceptibility testing and determination of MIC and MBC values for Ag-NPs.
- Scanning electron microscopy (SEM) to visualize bacterial morphology changes.
Main Results:
- 10% of the 120 samples were positive for C. pseudotuberculosis.
- A pan-drug-resistant strain of C. pseudotuberculosis was identified, resistant to six antibiotic classes.
- The MIC of Ag-NPs was 0.1 mg/ml (100 ppm) and the MBC was 0.05 mg/ml (50 ppm).
- SEM revealed significant morphological damage, including membrane rupture, in C. pseudotuberculosis treated with Ag-NPs.
Conclusions:
- Silver nanoparticles demonstrate potent antimicrobial activity against pan-drug-resistant C. pseudotuberculosis.
- Ag-NPs could serve as a novel therapeutic agent for treating caseous lymphadenitis pyogranulomas.
- This study highlights the potential of nanotechnology in combating antibiotic-resistant bacterial infections in livestock.
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