Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Editing02:23

RNA Editing

9.8K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.8K
RNA Interference01:23

RNA Interference

27.8K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.8K
Nucleic Acid Structure01:25

Nucleic Acid Structure

8.4K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
8.4K
RNA Splicing01:32

RNA Splicing

60.4K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.4K
Experimental RNAi02:15

Experimental RNAi

7.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.3K
Types of RNA01:20

Types of RNA

9.1K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
9.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Large-scale tethered screen of RNA-binding proteins reveals novel regulators of poly(A) site selection.

Molecular cell·2026
Same author

Comprehensive RNA-binding protein analyses and deep learning uncover genetic constraints and disease associations in protein-RNA interfaces.

Cell systems·2026
Same author

Flipper: An advanced framework for identifying differential RNA binding behavior with eCLIP data.

bioRxiv : the preprint server for biology·2026
Same author

Gas Bloat Syndrome after Nissen Fundoplication: Association with Anatomical Failure and Revisional Operation.

Journal of the American College of Surgeons·2026
Same author

Single-cell and isoform-specific translational profiling of the mouse brain.

Nature·2026
Same author

Dual-targeting snRNA gene therapy rescues STMN2 and UNC13A splicing in TDP-43 proteinopathies.

bioRxiv : the preprint server for biology·2026
Same journal

Cryo-EM sheds light on the mechanism of human telomerase inhibition by BIBR1532.

Nature chemical biology·2026
Same journal

Artificial metalloenzymes in complex biological environments.

Nature chemical biology·2026
Same journal

Allosteric disordering of eIF2B regulates the integrated stress response.

Nature chemical biology·2026
Same journal

A tail of two ligases.

Nature chemical biology·2026
Same journal

Non-canonical cytochrome P450 enzymes expand the diversity of bacterial hemoproteins.

Nature chemical biology·2026
Same journal

Image-guided activation of drugs with electromagnetic radiation.

Nature chemical biology·2026
See all related articles

Related Experiment Video

Updated: Jan 17, 2026

A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

2.9K

Enhancing RNA base editing on mammalian transcripts with small nuclear RNAs.

Aaron A Smargon1,2,3, Deepak Pant1,2,3,4, Trent A Gomberg1,2,3,5

  • 1Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA, USA.

Nature Chemical Biology
|September 18, 2025
PubMed
Summary
This summary is machine-generated.

Small nuclear RNAs (snRNAs) enhance RNA base editing and pseudouridylation for genetic disease therapies. Guided snRNAs offer a precise and persistent tool for RNA targeting, improving gene editing efficiency and safety.

More Related Videos

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
08:49

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

Published on: September 16, 2019

8.1K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.5K

Related Experiment Videos

Last Updated: Jan 17, 2026

A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

2.9K
Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
08:49

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

Published on: September 16, 2019

8.1K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.5K

Area of Science:

  • Molecular Biology
  • RNA Biology
  • Gene Therapy

Background:

  • Endogenous uridine-rich small nuclear RNAs (U snRNAs) are crucial for pre-mRNA processing.
  • Previous research established U snRNA's role in programmable exon splicing.
  • The potential of snRNAs to enhance RNA base editing remained unexplored.

Purpose of the Study:

  • To investigate if snRNAs can improve RNA base editing efficiency compared to existing technologies.
  • To explore the application of snRNAs for targeted RNA pseudouridylation.
  • To evaluate snRNA-based tools for treating genetic diseases like cystic fibrosis.

Main Methods:

  • Comparison of adenosine deaminase acting on RNA (ADAR)-recruiting circular RNAs with guided A>I snRNAs for adenosine-to-inosine editing.
  • Assessment of off-target gene perturbation and nuclear localization of snRNAs.
  • Engineering of snRNA-H/ACA box snoRNA fusions (U>Ψ snRNAs) for targeted pseudouridylation.
  • Utilizing a cystic fibrosis human bronchial epithelial cell model to assess CFTR rescue.

Main Results:

  • Guided A>I snRNAs demonstrated increased adenosine-to-inosine editing efficiency, particularly for higher exon count genes.
  • snRNAs showed reduced off-target gene effects and enhanced nuclear persistence compared to circular RNAs.
  • A>I snRNAs efficiently edited long noncoding RNAs and pre-mRNA 3' splice sites, promoting splicing alterations.
  • U>Ψ snRNAs facilitated targeted RNA pseudouridylation without DKC1 overexpression, leading to improved CFTR rescue from nonsense-mediated mRNA decay.

Conclusions:

  • snRNAs represent a powerful endogenous tool for enhancing RNA base editing and pseudouridylation.
  • snRNA-based approaches offer improved specificity and persistence for RNA targeting applications.
  • These findings advance RNA-targeting technologies for potential therapeutic applications in genetic diseases.