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Gene editing in cancer therapy: overcoming drug resistance and enhancing precision medicine

Hyeonjeong Park1, Suyeun Yu2, Taeyoung Koo3,4

  • 1Department of Regulatory Science, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea.

Cancer Gene Therapy
|September 19, 2025
PubMed

Insights

CRISPR gene editing offers precise cancer therapy by targeting oncogenes and enhancing treatments like CAR-T cells. Research is advancing rapidly, overcoming challenges for personalized cancer care.

Area of Science:

  • Biotechnology
  • Genetics
  • Oncology

Background:

  • CRISPR technology provides precise genetic manipulation capabilities.
  • Cancer gene therapy faces challenges like drug resistance and limited treatment efficacy.

Purpose of the Study:

  • To review the latest advancements in CRISPR applications for cancer therapy.
  • To explore CRISPR's potential in overcoming drug resistance and enhancing immunotherapies.
  • To discuss strategies for improving CRISPR safety and efficacy in cancer treatment.

Main Methods:

  • Review of current literature on CRISPR-based cancer therapeutics.
  • Analysis of CRISPR applications including gene knockout, base editing, and integration with other therapies.
  • Examination of oncolytic virus-mediated CRISPR delivery systems.

Main Results:

  • CRISPR demonstrates potential in targeted oncogene disruption, mutation correction, and immune modulation.
  • CRISPR enhances chemotherapy sensitivity and immunotherapy strategies (e.g., CAR-T cells).
  • Oncolytic virus delivery shows promise for tumor-specific gene editing with minimized off-target effects.

Conclusions:

  • CRISPR-based cancer therapeutics are progressing from preclinical research to clinical trials.
  • Key challenges include off-target effects, immune responses, and delivery limitations.
  • CRISPR holds significant potential for developing personalized cancer treatments.

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