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LncRNA DGUOK-AS1 Promotes Bladder Cancer Progression by Enhancing EFTUD2 Stability
Wenwei Ying1, Dingliang Li2, Ninghan Feng3
1Department of Urology, Peking University First Hospital, Beijing, China.
Cancer Research and Treatment
|September 19, 2025
Summary
Long noncoding RNA DGUOK-AS1 promotes bladder cancer progression by enhancing cell proliferation and invasion. This lncRNA targets the EFTUD2/MST1R/Akt pathway, presenting a potential therapeutic strategy for bladder cancer.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long noncoding RNAs (lncRNAs) play critical roles in cancer, but their mechanisms are often unclear.
- Understanding lncRNA functions is vital for cancer research and therapeutic development.
Purpose of the Study:
- To identify differentially expressed lncRNAs in bladder cancer.
- To investigate the functional role and mechanism of lncRNA DGUOK-AS1 in bladder cancer progression.
Main Methods:
- RNA sequencing to compare lncRNAs in non-muscle-invasive bladder cancer (NMIBC) and muscle-invasive bladder cancer (MIBC).
- In vitro and in vivo assays to assess DGUOK-AS1 function.
- Molecular assays (RNA pull-down, RIP, co-IP, Western blot, RT-qPCR) to elucidate DGUOK-AS1 regulatory mechanisms.
Main Results:
- lncRNA DGUOK-AS1 was significantly upregulated in MIBC compared to NMIBC.
- DGUOK-AS1 promotes bladder cancer cell proliferation and invasion by interacting with EFTUD2.
- DGUOK-AS1 stabilizes EFTUD2 by preventing its ubiquitination and degradation, leading to RON∆165 isoform production and Akt/PKB pathway activation.
Conclusions:
- DGUOK-AS1 drives bladder cancer progression through the EFTUD2/MST1R/Akt signaling axis.
- DGUOK-AS1 represents a promising therapeutic target for bladder cancer treatment.
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