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PagARF3.1 promotes adventitious root formation by repressing IPT-mediated cytokinin biosynthesis.
Ying-Li Liu1, Xue-Qin Song2,3, Hui He2
1State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.
Forestry Research
|September 19, 2025
Summary
PagARF3.1 positively regulates adventitious root formation in hybrid poplar. This gene represses cytokinin biosynthesis, promoting faster and more abundant root development crucial for plant propagation.
Area of Science:
- Plant Biology
- Molecular Biology
- Genetics
Background:
- Adventitious root formation is vital for clonal propagation in horticultural and woody species.
- Auxin and cytokinin antagonistically regulate root development, but molecular mechanisms are unclear.
Purpose of the Study:
- Investigate the role of PagARF3.1, an auxin response factor homolog, in hybrid poplar adventitious root formation.
Main Methods:
- GUS staining to analyze gene expression patterns.
- RNA interference (RNAi) and overexpression to study gene function.
- Real-time quantitative PCR to assess gene expression.
- Yeast one-hybrid and ChIP-PCR to confirm direct gene binding.
Main Results:
- PagARF3.1 expression is localized in developing adventitious roots.
- Knockdown of PagARF3.1 delayed rooting and reduced biomass; overexpression promoted rooting.
- PagARF3.1 directly represses the expression of PagIPT5a and PagIPT5b, key genes in cytokinin biosynthesis.
Conclusions:
- PagARF3.1 acts as a positive regulator of adventitious root formation in hybrid poplar.
- The mechanism involves repressing IPT-mediated cytokinin biosynthesis.
- This finding provides insights into molecular regulation of plant root development.
