Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.7K
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

18.8K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
18.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Creation and manipulation of bipartite expression transgenes in C. elegans using phiC31 recombinase.

Genetics·2026
Same author

Parameters that influence bipartite reporter system expression in Caenorhabditis elegans.

Genetics·2025
Same author

F-box protein FBXB-65 regulates anterograde transport of the kinesin-3 motor UNC-104 through a PTM near its cargo-binding PH domain.

Journal of cell science·2024
Same author

Dominant negative variants in KIF5B cause osteogenesis imperfecta via down regulation of mTOR signaling.

PLoS genetics·2023
Same author

UNC-49 is a redox-sensitive GABA<sub>A</sub> receptor that regulates the mitochondrial unfolded protein response cell nonautonomously.

Science advances·2023
Same author

Rapid generation of Caenorhabditis elegans single-copy transgenes combining recombination-mediated cassette exchange and drug selection.

Genetics·2023

Related Experiment Video

Updated: Jan 17, 2026

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

17.0K

A single component landing site for efficient transgenesis using recombination-mediated insertion.

Aiden A Beck1, Michael L Nonet1

  • 1Dept. of Neuroscience, Washington University Medical School.

Micropublication Biology
|September 19, 2025
PubMed
Summary
This summary is machine-generated.

A new recombination-mediated insertion (RMI) method simplifies creating C. elegans transgenes. This approach uses a specific landing site and targeting vectors for efficient genetic engineering in C. elegans.

More Related Videos

Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

19.7K
Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus
11:36

Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus

Published on: November 20, 2016

10.2K

Related Experiment Videos

Last Updated: Jan 17, 2026

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

17.0K
Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

19.7K
Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus
11:36

Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus

Published on: November 20, 2016

10.2K

Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • Recombination-mediated insertion (RMI) is an emerging technique for generating transgenes in *C. elegans*.
  • The existing RMI method relies on a two-component system involving an *att* landing site and a separate source of phiC31 and FLP recombinases.

Purpose of the Study:

  • To describe a novel landing site that integrates the recombinase cassette for recombination-mediated insertion.
  • To present matching targeting vectors for use with the new landing site.
  • To demonstrate the efficiency of this RMI system in *C. elegans*.

Main Methods:

  • Development of a new landing site integrated with recombinase expression cassettes.
  • Design of complementary targeting vectors for recombination-mediated insertion.
  • Integration of the landing site into a well-characterized MosSCI insertion locus on Chromosome IV in *C. elegans*.
  • Generation of multiple independent transgene insertions via microinjection into single animals.

Main Results:

  • The new landing site, located on Chr IV, facilitates efficient recombination-mediated insertion.
  • Microinjections into single animals typically resulted in multiple independent transgene insertions.
  • Fourteen distinct fluorescent reporters targeting pharyngeal gland cells were successfully generated using this RMI system.

Conclusions:

  • The described RMI landing site and targeting vectors provide an efficient and robust method for *C. elegans* transgene creation.
  • This system simplifies the generation of multiple independent insertions, particularly for complex reporter constructs.
  • The successful generation of pharyngeal gland cell reporters highlights the utility of this approach for studying gene expression and function in *C. elegans*.