Rapid DNA/eDNA-Based ID Tools for Improved Chondrichthyan Monitoring and Management.

Area of Science:

• Conservation genetics
• Molecular ecology
• Biodiversity monitoring

Background:

• Rapid DNA/eDNA identification tools are vital for monitoring biodiversity and wildlife trade, yet their application is hindered by limited standardized protocols and taxonomic coverage.
• Chondrichthyes (sharks and rays) are heavily exploited, with significant data scarcity hindering conservation assessments and the development of molecular identification tools.

Purpose of the Study:

• To synthesize the current state of rapid DNA/eDNA-based identification tools for chondrichthyan species.
• To compile available taxon-specific primers and recommend suitable methods for species identification.
• To identify gaps in taxonomic and geographic coverage and emphasize the need for further research.

Main Methods:

• Literature review of established and emerging rapid DNA/eDNA-based identification methods.
• Compilation and analysis of available taxon-specific primers for chondrichthyan species.
• Assessment of current taxonomic and geographic coverage of these tools.

Main Results:

• The review synthesizes current rapid DNA/eDNA tools for chondrichthyan species identification.
• Key gaps in taxonomic and geographic coverage were identified, particularly for under-represented species and regions.
• A compilation of available primers and recommendations for suitable methods are provided.

Conclusions:

• Further research is needed to expand the coverage of rapid DNA/eDNA tools to under-represented chondrichthyan species and regions.
• Integrating genetic approaches into enforcement frameworks is crucial for enhancing conservation strategies and regulatory compliance.
• This work provides a reference to support evidence-based decision-making for the conservation and management of Chondrichthyes species worldwide.