Kinetic-Controlled Synthesis of Walnut-like Core-Shell Magnetic Mesoporous Silica Microspheres for Enhanced Enzyme...

Area of Science:

• Materials Science
• Biocatalysis
• Nanotechnology

Background:

• Magnetic mesoporous materials offer significant potential in biomedicine, catalysis, and environmental applications.
• Efficient immobilization of enzymes like lipase is crucial for biocatalytic processes.

Purpose of the Study:

• To develop a novel core-shell nanocarrier (CS-WMM) for enhanced lipase immobilization and phytosterol ester synthesis.
• To investigate the structure-property relationships of the developed magnetic mesoporous material.

Main Methods:

• Surfactant-free kinetic-controlled interfacial assembly was used to construct walnut-like dual-mesoporous microspheres (inner flower-like MnO2, outer mesoporous SiO2).
• Characterization of pore size (4.8 nm) and surface area (158.61 m2/g).
• Lipase immobilization and assessment of esterification conversion and enzyme activity retention.

Main Results:

• The CS-WMM nanocarrier demonstrated a high enzyme loading capacity (210 mg g-1) and improved substrate diffusion.
• Achieved 78.33% esterification conversion and retained 55.03% activity after 7 cycles.
• Outperformed Fe3O4@MnO2 in terms of catalytic efficiency and stability.

Conclusions:

• The developed CS-WMM material provides a promising strategy for designing carriers with high loading capacity and structural stability.
• This magnetic mesoporous material is suitable for hydrophobic substrate-driven biocatalytic systems.
• Potential applications include food lipid processing and biopharmaceuticals.