Kinetic-Controlled Synthesis of Walnut-like Core-Shell Magnetic Mesoporous Silica Microspheres for Enhanced Enzyme...

Magnetic mesoporous materials, integrating magnetic nanoparticles and mesoporous structures, have great potential in biomedicine, catalysis, and the environment. This study develops a novel core-shell nanocarrier (CS-WMM: magnetic core-flower-like MnO₂ mesoporous layer-silica shell) for efficient lipase immobilization and enhanced phytosterol esters synthesis. Via a surfactant-free kinetic-controlled interfacial assembly, walnut-like dual-mesoporous microspheres (inner flower-like MnO₂, outer mesoporous SiO₂: 4.8 nm pore, 158.61 m²/g surface) are constructed, enabling a high enzyme loading (210 mg g^-1) and improved substrate diffusion. Compared with Fe₃O₄@MnO₂, CS-WMM achieves 78.33% esterification conversion and retains 55.03% activity after 7 cycles via dual-mesoporous synergy. This work provides a novel material design strategy with high loading capacity and structural stability for hydrophobic substrate-driven biocatalytic systems, which holds potential applications in food lipid processing, biopharmaceuticals, and other fields.