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Updated: Jan 17, 2026
Author Spotlight: Peptidome Extraction from Small Extracellular Vesicles Isolated from Bone Marrow-Derived Macrophages
Published on: June 30, 2023
Protocol for isolating and characterizing extracellular vesicles by ultracentrifugation from bone marrow-derived
Yuxi Chen1, Runmin Ding1, Jinxu Miao1
1Department of Urology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210011, China.
None:
Extracellular vesicles (EVs) enable the transmission of crucial molecular components between the parental and recipient cells. Macrophages can polarize into two distinct macrophage phenotypes, thereby exerting diverse effects on recipient cells. Here, we present a protocol for the direct isolation of EVs from bone marrow-derived macrophages (BMDMs) using ultracentrifugation. We describe steps for culturing BMDMs and macrophage polarization. We then detail procedures for further characterizing the isolated EVs using transmission electron microscopy (TEM), western blotting, and nanoparticle tracking analysis (NTA).
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