Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

6.9K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
6.9K
Non-vascular Seedless Plants02:26

Non-vascular Seedless Plants

70.9K
The diverse plant life on Earth—consisting of nearly 400,000 species—can be divided into three broad categories based on biological characteristics: nonvascular, seedless vascular, and seed plants.
70.9K
Asexual Reproduction02:38

Asexual Reproduction

37.1K
Asexual reproduction allows plants to reproduce without growing flowers, attracting pollinators, or dispersing seeds. Offspring are genetically identical to the parent and produced without the fusion of male and female gametes.
37.1K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

20.5K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
20.5K
Seedless Vascular Plants03:24

Seedless Vascular Plants

66.6K
Seedless Vascular Plants Were the First Tall Plants on Earth
66.6K
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

9.0K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anthocyanins and betalains in evolution: Why mutual exclusion remains the best-supported model.

Plant biology (Stuttgart, Germany)·2026
Same author

Replication-stress-induced chromatin loops protect fork stability.

Nature·2026
Same author

Conserved and Lineage-Specific Roles of KEA-Mediated Ion Homeostasis in Chlamydomonas.

Plant physiology·2026
Same author

Emerging technologies for the discovery of biosynthetic genes in plants.

Natural product reports·2026
Same author

Deep tree roots at risk of accelerating groundwater pollution beneath clay-rich aquitards.

Nature communications·2026
Same author

Extensive variation between chromosomes of North American and European hop.

Nature communications·2026
Same journal

Efficient CRISPR-Cas9 delivery and transgene-free multiplex genome editing in plants using cymbidium mosaic virus-derived vectors.

The Plant journal : for cell and molecular biology·2026
Same journal

Hypsochromic shift in phytochrome C Pr absorption complements phytochrome B-mediated inhibition of hypocotyl elongation.

The Plant journal : for cell and molecular biology·2026
Same journal

The HD-Zip IV gene ZmHB118 is required for basal endosperm transfer layer formation and maternal-to-filial nutrient allocation during maize seed filling.

The Plant journal : for cell and molecular biology·2026
Same journal

An ER-microtubule bridge: Reticulon 17 links microtubules with ER network organisation in plants.

The Plant journal : for cell and molecular biology·2026
Same journal

Chromosome-scale genome of Myriophyllum spicatum unveils the role of allohexaploidy in driving aquatic adaptation and widespread invasion.

The Plant journal : for cell and molecular biology·2026
Same journal

Rapid CO<sub>2</sub> effects on protein-related metabolism during photosynthetic gas exchange.

The Plant journal : for cell and molecular biology·2026
See all related articles

Related Experiment Video

Updated: Jan 17, 2026

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
13:03

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

11.0K

Assembling genomes of non-model plants: A case study with evolutionary insights from Ranunculus (Ranunculaceae).

Kevin Karbstein1,2,3, Nancy Choudhary4, Ting Xie5

  • 1Albrecht-von-Haller Institute for Plant Sciences, Department of Systematics, Biodiversity and Evolution of Plants (with Herbarium), University of Göttingen, Göttingen, Germany.

The Plant Journal : for Cell and Molecular Biology
|September 19, 2025
PubMed
Summary
This summary is machine-generated.

Researchers generated high-quality genome sequences for Ranunculus, overcoming challenges in sequencing large plant genomes. This work provides crucial genomic resources for evolutionary studies in the Ranunculaceae family.

Keywords:
Illumina vs. Nanopore vs. PacBio sequencingRanunculaceaeRanunculus auricomus species complexde novo assembly strategiesgene evolutionlarge non‐model plant genomesmitogenomenuclear genomeplastome

More Related Videos

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
10:40

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

10.9K
Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
08:09

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

20.5K

Related Experiment Videos

Last Updated: Jan 17, 2026

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
13:03

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

11.0K
Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
10:40

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

10.9K
Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
08:09

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

20.5K

Area of Science:

  • Plant genomics
  • Evolutionary biology
  • Bioinformatics

Background:

  • Genome sequencing is crucial for understanding plant evolution, but large and complex genomes pose significant challenges.
  • The genus Ranunculus (Ranunculaceae) is evolutionarily important for studying polyploidy and reticulate evolution, yet lacks high-quality genome sequences.
  • Previous genomic studies in Ranunculaceae have been limited by the absence of essential nuclear and mitochondrial genome data.

Purpose of the Study:

  • To develop and evaluate genome assembly strategies for plant species with large, complex genomes.
  • To generate high-quality plastome, mitogenome, and nuclear genome sequences for a key species in the Ranunculus genus.
  • To provide a foundation for advanced phylogenomic, functional, and taxonomic analyses within Ranunculaceae.

Main Methods:

  • Comparative analysis of Illumina short-read, Oxford Nanopore Technology (ONT) long-read, and PacBio (HiFi) long-read sequencing and assembly strategies.
  • Sequencing of the diploid progenitor species R. cassubicifolius.
  • Assembly and annotation of plastome, mitogenome, and nuclear genomes, including scaffolding using chromatin conformation (Hi-C) data.

Main Results:

  • Successful assembly of a 156 kbp plastome and a 1.18 Mbp mitogenome using hybrid strategies.
  • Generation of a 2.69 Gbp haploid nuclear genome sequence with high completeness (94.1% BUSCO) and 35,482 annotated genes.
  • Phylogenomic analysis of Ranunculaceae based on updated plastome and novel mitogenome data, including insights into gene loss.
  • Inference of ancient gene duplications in Ranunculales by comparing with existing genomes.

Conclusions:

  • The developed genome sequencing and assembly strategies are effective for large, complex plant genomes.
  • The generated genomic resources for Ranunculus will significantly advance evolutionary and functional studies within the Ranunculaceae family.
  • This study provides a critical foundation for future research on angiosperm evolution, polyploidy, and adaptation.