TLC Method Assisted by Digital Images for Analysis of Ivermectin-Based Product.

Area of Science:

• Analytical Chemistry
• Green Chemistry
• Pharmaceutical Analysis

Background:

• Ivermectin (IVE) is a widely used antiparasitic agent available in various formulations.
• Existing analytical methods for ivermectin lack environmental friendliness and efficiency.
• There is a need for green analytical approaches to assess ivermectin products.

Purpose of the Study:

• To develop and validate an eco-efficient, rapid, and cost-effective method for analyzing ivermectin injectable solutions.
• To utilize thin-layer chromatography (TLC) combined with digital image analysis for ivermectin quantification.
• To assess the greenness of the developed analytical method using established metrics.

Main Methods:

• Employing silica gel plates and an ethyl acetate:ethanol mobile phase for TLC separation.
• Utilizing Image J software to analyze pixel data from UV-visualized spots.
• Performing forced degradation studies to assess method stability.
• Quantifying ivermectin in commercial injectable solutions.

Main Results:

• The developed TLC method demonstrated selectivity, stability, linearity (100-900 µg/mL), precision (RSD < 2%), accuracy, and ruggedness.
• The method successfully quantified commercial ivermectin products with an average content of 98.95%.
• Greenness assessment using NEMI, ESA, AGREE, and GAPI indicated a favorable environmental profile.

Conclusions:

• The developed thin-layer chromatography method, assisted by digital image analysis, is selective, stable, linear, precise, accurate, and rugged for ivermectin quantification in injectable solutions.
• The method is recognized as 'green' based on multiple analytical greenness assessment tools.
• This approach offers an efficient and environmentally conscious alternative for ivermectin analysis.