Single-cell Omics Assessment of Mitochondrial Function: Current Status and Future Perspectives.

Area of Science:

• Biochemistry
• Genomics
• Cell Biology

Background:

• Mitochondria play crucial roles in cellular energy production and homeostasis.
• Mitochondrial dysfunction is implicated in numerous diseases.
• Single-cell omics technologies provide unprecedented resolution for studying cellular heterogeneity.

Purpose of the Study:

• To review advancements in single-cell omics for studying mitochondria.
• To categorize mitochondrial dysfunction and variability in pathological and physiological states.
• To highlight the applications and future directions of single-cell mitochondrial analysis.

Main Methods:

• Review of cutting-edge single-cell omics technologies.
• Analysis of studies on mitochondrial function and dysfunction.
• Integration of genomic and phenomic data at the single-cell level.

Main Results:

• Single-cell omics effectively address intracellular and intercellular heterogeneity in mitochondria.
• Categorization of mitochondrial dysfunction and variability across diverse contexts.
• Demonstration of single-cell mitochondrial assessment applications in disease and therapeutics.

Conclusions:

• Single-cell omics are revolutionizing mitochondrial research.
• These technologies offer potential for novel therapeutic strategies for mitochondrial diseases.
• Future research should focus on overcoming current methodological limitations.