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Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
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Two-photon lithography-fabricated deterministic lateral displacement microfluidic system for efficient minicell

Sharaj Hegde Sharavu1, Sagar Bhagwat1, Sebastian Kluck1

  • 1NeptunLab - Laboratory of Process Technology, Department of Microsystems Engineering (IMTEK), University of Freiburg, Georges-Köhler- Allee 103, Freiburg, 79110, Germany.

Biomedical Microdevices
|September 20, 2025
PubMed
Summary
This summary is machine-generated.

This study uses microfluidics to purify chromosome-less minicells for cancer drug delivery. Deterministic Lateral Displacement (DLD) systems achieved 94% separation efficiency, overcoming a key challenge in minicell purification.

Keywords:
Deterministic lateral displacementMicrofluidicsMinicell purificationTwo-photon lithography

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Nanotechnology

Background:

  • Chromosome-less minicells show promise as nanocarriers for targeted cancer drug delivery.
  • Purifying minicells from larger parental cells presents a significant challenge due to size and shape differences.

Purpose of the Study:

  • To investigate Deterministic Lateral Displacement (DLD) microfluidic systems for efficient minicell purification.
  • To leverage Two-Photon Lithography (TPL) for rapid prototyping of high-resolution microfluidic devices.

Main Methods:

  • Investigated DLD design parameters like post gaps and outlet widths under laminar flow.
  • Developed a two-stage system combining a spiral inertial chip and an optimized DLD chip.
  • Fabricated chips using high-resolution TPL for precise microfluidic designs.

Main Results:

  • Achieved a 94% separation efficiency for minicells from parental cells.
  • Demonstrated the effectiveness of a two-stage microfluidic system for size and shape-based cell sorting.
  • Optimized asymmetric DLD chip with a 2 μm downstream post gap.

Conclusions:

  • Advanced microfluidic systems, particularly DLD, are highly effective for purifying minicells.
  • This purification method enhances the potential of minicells as nanocarriers in cancer therapy.
  • Microfluidics offers a powerful tool for cell sorting based on distinct physical characteristics.