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Updated: Jan 17, 2026
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
Sensitive constricted lateral flow immunoassay for 4-chlorophenoxyacetic acid detection based on monoclonal antibody
Peiyuan Cai1, Shuzhen Huang1, Yangyang Zhang1
1Key Laboratory of Modern Analytical Science and Separation Technology of Fujian Province, Key Laboratory of Pollution Monitoring and Control of Fujian Provincial Universities, College of Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou 363000, China.
None:
To meet the urgent need for rapid on-site assay of 4-chlorophenoxyacetic acid (4-CPA), herein, a monoclonal antibody (mAb) against 4-CPA was screened via hybridoma. The mAb demonstrated the highest affinity of 2.39 × 1010 L/mol to date. ELISA exhibited an IC50 of 11.64 ng/mL, a linear range of 2.95-58.32 ng/mL, and a limit of detection (LOD) of 0.84 ng/mL. For rapid on-site assays within 15 min, a colloidal gold strip assay was established with a detection threshold of 400 ng/mL and a visual LOD of 50 ng/mL. To further improve sensitivity, an original strategy was provided via constricting the widths of Test and Control zones, and this improved assay had a threshold of 200 ng/mL and a visual LOD of 12.5 ng/mL. The results in bean sprout samples were consistent with LC-MS/MS findings. Therefore, the mAb and developed assays had great potential for on-site monitoring of 4-CPA in actual samples.
