Related Experiment Video
Updated: Jan 17, 2026

Author Spotlight: Generation of Patient-Derived Podocytes from Skin Biopsies
Published on: May 26, 2023
iPSC line DHMCi010-A is derived from a hereditary nephrotic syndrome patient with an autosomal recessive NPHS2
Robert Matthes1, Mansoureh Tabatabaeifar1, Karin Burau2
1Center for Child and Adolescent Medicine, Department of Pediatric Nephrology, University Hospital Heidelberg, Heidelberg, Germany.
Abstract:
Recessive mutations in the NPHS2 gene, encoding the podocyte membrane protein podocin, are the most common genetic cause of childhood-onset nephrotic syndrome. To generate induced pluripotent stem cells (iPSCs), peripheral blood mononuclear cells (PBMCs) from a male patient with a homozygous NPHS2 mutation (c.413G>A(;)(c.413G>A)) were reprogrammed using the Cytotune®-iPSC 2.0 Sendai Reprogramming Kit (Invitrogen). The resulting iPSCs exhibit normal morphology and karyotype, express undifferentiated hPSC state markers, and demonstrate the capacity for spontaneous differentiation into all three germ layers in vitro.
More Related Videos
09:37Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
Published on: March 5, 2017
08:46Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
Published on: September 1, 2015
Related Concept Videos
iPS Cell Differentiation
Nephrotic Syndrome I : Introduction
EPS and iPS Cells in Disease Research
Nephrotic Syndrome III : Nursing Management
Nephrotic Syndrome II : Assessment and Medical Management