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Updated: Jul 29, 2026
07:58
A Polyaniline-based Sensor of Nucleic Acids
Published on: November 1, 2016
8.4K
Development of a highly efficient DNA-based biosensor using a functionalized MoS₂/polypyrrole nanocomposite for
Afsaneh Mousa Pour1, Zahra Garkani-Nejad2, Hadi Mahmoudi-Moghaddam3
1Chemistry Department, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran; Young Researchers Society, Shahid Bahonar University of Kerman, Kerman, Iran.
Bioelectrochemistry (Amsterdam, Netherlands)
|September 20, 2025
Summary
Researchers developed a novel electrochemical biosensor using europium-doped molybdenum disulfide nanoparticles and polypyrrole for detecting mitomycin C. This DNA biosensor shows high sensitivity and potential for environmental and biomedical monitoring.
Area of Science:
- Nanomaterials Science
- Electrochemistry
- Biosensor Technology
Background:
- Mitomycin C (MC) is a crucial chemotherapeutic agent with significant toxicity.
- Sensitive and reliable detection methods for MC are essential for therapeutic drug monitoring and environmental safety.
- Existing detection methods often lack the required sensitivity, selectivity, or practicality for real-world applications.
Purpose of the Study:
- To synthesize novel plate-like Europium (Eu³⁺)-doped Molybdenum Disulfide (MoS₂) nanoparticles (PL-Eu³⁺-doped MoS₂ NPs).
- To develop a sensitive electrochemical DNA biosensor for detecting mitomycin C (MC) using the synthesized nanocomposite.
- To investigate the interaction mechanism between double-stranded DNA (ds-DNA) and MC.
Main Methods:
- Hydrothermal synthesis of PL-Eu³⁺-doped MoS₂ NPs.
- Fabrication of an electrochemical biosensor by modifying pencil graphite electrodes (PGEs) with PL-Eu³⁺-doped MoS₂ NPs and polypyrrole (PPy).
- Electrochemical analysis using differential pulse voltammetry (DPV), UV/Vis spectrophotometry, and molecular docking to study ds-DNA/MC interaction.
Main Results:
- The PL-Eu³⁺-doped MoS₂/PPy nanocomposite was successfully synthesized and characterized.
- The developed ds-DNA/PL-Eu³⁺-doped MoS₂/PGE biosensor demonstrated a linear detection range for MC from 1 to 75 μg/mL.
- A low limit of detection (LOD) of 0.8 μg/mL was achieved, with excellent recovery rates (97–104%) in real sample analyses.
- Molecular docking and spectroscopic data confirmed groove binding as the primary interaction mode between MC and ds-DNA.
Conclusions:
- The novel electrochemical DNA biosensor based on PL-Eu³⁺-doped MoS₂/PPy nanocomposite offers a sensitive and reliable platform for MC detection.
- The sensor exhibits excellent performance, making it suitable for practical applications in biomedical and environmental monitoring.
- The study provides valuable insights into the interaction mechanism between MC and DNA, aiding in understanding its biological activity.
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