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Updated: Jul 18, 2026
Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes
Published on: September 17, 2012
Protocol for Hi-C-based identification of chromosomal inversions in mosquitoes
Varvara Lukyanchikova1, Ilya I Brusentsov2, Dmitry A Karagodin2
1Department of Entomology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA; Fralin Life Sciences Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
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Chromosomal inversions play an important role in the genomic evolution and adaptation of mosquito species. Here, we present a protocol for detecting chromosomal inversions in mosquitoes using Hi-C technology and chromatin contact heatmaps. We describe the steps for Hi-C library preparation using the Hi-C Arima+ kit, along with the Arima Library Prep Module, and provide several adaptations for mosquito samples. We then outline procedures for Hi-C data analysis, Hi-C heatmap generation, and the identification of polymorphic and fixed inversions. For additional details on the use and execution of this protocol, please refer to Lukyanchikova et al.1.
