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Comprehensive proteomic characterization and functional annotation of common carp seminal plasma
Anna Malgorzata Majewska1, Natalia Kodzik1, Mariola Aleksandra Dietrich1
1Institute of Animal Reproduction and Food Research, PAS, Trylinskiego 18, 10-683 Olsztyn, Poland.
Reproduction, Fertility, and Development
|September 22, 2025
Summary
This study identified 1402 proteins in carp seminal plasma, revealing key roles in reproduction and aquaculture. The comprehensive proteomic profile enhances understanding of fish reproductive biology and potential applications.
Area of Science:
- Aquaculture and Reproductive Biology
- Proteomics and Bioinformatics
Background:
- Seminal plasma protein composition is vital for fish reproduction and aquaculture.
- Limited comprehensive proteomic data exists for carp seminal plasma.
Purpose of the Study:
- To comprehensively characterize the carp seminal plasma proteome.
- Classify identified proteins by cellular localization and function.
- Explore roles in reproductive and physiological processes.
Main Methods:
- High-throughput liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomic analysis.
- Utilized updated Cyprinus carpio genome annotation for protein identification and classification.
- Employed bioinformatic tools for functional and pathway analysis.
Main Results:
- Identified a record 1402 proteins in carp seminal plasma.
- Classified proteins based on homology to human genes and fish-specific annotations.
- Discovered fish-specific proteins involved in immunity, detoxification, and stress response; identified proteins linked to reproduction and extracellular vesicles.
Conclusions:
- This study provides the most extensive proteomic profile of carp seminal plasma to date.
- Offers novel insights into carp reproductive biology and seminal plasma functions.
- Findings have implications for aquaculture, including sperm preservation and disease resistance.
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