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Related Experiment Video

Updated: Jan 17, 2026

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On-chip oocyte cumulus removal using vibration-induced flow.

Amirhossein Favakeh1, Amir Mokhtare1,2, Hanxue Zhang3

  • 1Department of Food Science, College of Agriculture and Life Sciences (CALS), Cornell University, Ithaca, New York, 14853 USA. alireza@cornell.edu.

Lab on a Chip
|September 22, 2025
PubMed
Summary
This summary is machine-generated.

Cumulus removal (CR), essential for assisted reproductive technologies (ART), is now automated. A novel platform using vibration-induced flow (VIF) efficiently removes cumulus cells from oocytes, ensuring consistent results without impacting fertilization rates.

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Area of Science:

  • Biotechnology
  • Reproductive Biology
  • Bioengineering

Background:

  • Cumulus removal (CR) is a critical step in assisted reproductive technologies (ART).
  • Traditional CR methods, like manual pipetting, can introduce variability and inconsistency.
  • There is a need for automated and standardized CR techniques in ART.

Purpose of the Study:

  • To develop and evaluate an open-surface platform for automated cumulus removal (CR) from oocytes.
  • To investigate the efficacy of vibration-induced flow (VIF) for CR.
  • To compare the performance of VIF-based CR with traditional manual methods in terms of oocyte survival and developmental potential.

Main Methods:

  • An open-surface platform utilizing pillars and vibration-induced flow (VIF) was designed for particle and cumulus cell separation.
  • The CR process was optimized by adjusting actuation power, time, and hyaluronidase (HA) concentration.
  • Mouse cumulus-oocyte complexes (COCs) underwent CR using either VIF or manual pipetting, followed by insemination and assessment of fertilization and blastocyst rates.

Main Results:

  • The VIF platform demonstrated efficient separation of small particles, removing 99% from the loading chamber.
  • Simultaneous CR of up to 23 oocytes was achieved without any oocyte loss.
  • No statistically significant differences were observed in fertilization (90.7% manual vs. 93.1% VIF) or blastocyst rates (50.0% manual vs. 43.1% VIF) between VIF and manual CR methods.

Conclusions:

  • The developed VIF platform effectively automates cumulus removal (CR) in a high-throughput manner.
  • This technology reduces manual labor and enhances standardization in assisted reproductive technologies (ART).
  • VIF-based CR is a viable alternative to manual methods, maintaining oocyte quality and developmental potential.