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Graphene Oxide-Activated Cellular Ceramic Composite Monoliths for Protein Purification.

Eleonora Lalli1, Riccardo Onesti1, Andrei V Kovalevsky2

  • 1Dipartimento di Ingegneria Civile, Chimica, Ambientale e dei Materiali, DICAM, Alma Mater Studiorum-Università di Bologna, via Terracini 28, Bologna 40131, Italy.

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New ceramic monoliths show promise for large-scale protein purification. These materials offer excellent flow properties, making them efficient for bioseparation processes like chromatography.

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Area of Science:

  • Materials Science
  • Chemical Engineering
  • Biotechnology

Background:

  • Chromatographic stationary phases are crucial for bioseparation.
  • Developing materials with optimal flow properties and binding capacity is essential for efficient protein purification.

Purpose of the Study:

  • To prepare and evaluate Al2TiO5-Al2O3 composite ceramic monoliths as a stationary phase for process chromatography.
  • To characterize the porous structure and flow properties of the ceramic monoliths.
  • To assess the performance of functionalized monoliths for bovine serum albumin (BSA) binding capacity.

Main Methods:

  • Preparation of Al2TiO5-Al2O3 composite ceramic monoliths.
  • Characterization of pore size distribution and hydraulic permeability.
  • Pulse tests for interstitial porosity, axial dispersion coefficient, and height equivalent to a theoretical plate.
  • Surface activation with graphene oxide and functionalization with Cibacron Blue F3GA.
  • Dynamic binding capacity measurements using BSA.

Main Results:

  • The ceramic monoliths exhibited a well-defined porous structure.
  • High permeability and convection were observed, indicating favorable flow properties for bioseparations.
  • The functionalized columns demonstrated dynamic binding capacity for BSA.

Conclusions:

  • Al2TiO5-Al2O3 composite ceramic monoliths possess advantageous flow characteristics for chromatographic bioseparations.
  • The material's convective properties are promising for protein purification applications.
  • Further optimization of the functionalization process could lead to highly efficient convective media for protein purification.