Extraction Optimization and Characterization of Antioxidant Polysaccharides From Suillus bovinus
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View abstract on PubMed
Summary
This summary is machine-generated.Researchers optimized crude polysaccharide extraction from Suillus bovinus using Box-Behnken design. The Y-1 fraction showed significant antioxidant potential in cell models, indicating its promise as a natural antioxidant.
Area Of Science
- Natural Product Chemistry
- Food Science
- Biochemistry
Background
- Polysaccharides from edible fungi possess various bioactivities.
- Suillus bovinus is a mushroom with potential health benefits.
Purpose Of The Study
- To optimize the extraction of crude polysaccharides (SubPs) from Suillus bovinus.
- To evaluate the antioxidant activity of purified polysaccharide fractions.
Main Methods
- Box-Behnken design combined with response surface methodology (BBD-RSM) for extraction optimization.
- DEAE-52 cellulose and Sephadex G-100 chromatography for purification.
- In vitro antioxidant assays using HepG2 cells and biochemical analyses.
Main Results
- Optimized extraction parameters yielded SubPs.
- Four homogeneous fractions (S-1, S-2, Y-1, Y-2) were obtained with molecular weights ranging from 47.34 to 97.07 kDa.
- Fraction Y-1 demonstrated significant antioxidant activity by reducing reactive oxygen species (ROS) and malondialdehyde (MDA) levels, and enhancing antioxidant enzyme activities (SOD, CAT, GSH-Px).
Conclusions
- The study successfully optimized polysaccharide extraction from Suillus bovinus.
- Fraction Y-1 exhibits potent antioxidant properties, supported by in vitro cell-based assays and structural characterization.
- Suillus bovinus polysaccharides, particularly fraction Y-1, show potential as natural antioxidants for health applications.
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