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Updated: Apr 28, 2026
Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model
Published on: September 3, 2020
Temporal regulation of genetic programs governing multiple cell death during myocardial ischemia-reperfusion injury
Qiuyu Pang1, Xiangmin Meng1, Zhenfang Zhou1
1Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, China.
Introduction:
Reperfusion serves as an effective therapeutic strategy for myocardial infarction (MI), but it causes damage to the heart. Although many studies have investigated the mechanism of disparate forms of cell death in myocardial ischemia-reperfusion injury (I/R), there remains a paucity of studies focus on the direct comparison of the mode of cell death events resulting from different reperfusion periods.
Methods:
We conducted an analysis of different sequencing data available in public databases to investigate the relationship between the diverse patterns of cell death and different reperfusion times. Additionally, we evaluated the time window of multiple categories of cell death between cells and mice.
Results:
We explored the relationship between the various modes of cell death and different reperfusion times induced by 6h, 12h and 24 h reperfusion. Our findings revealed that apoptosis occurred in the early stage of I/R injury and continued to appear as the reperfusion time increased. Meanwhile, the changes in autophagy and cuproptosis were also more obvious in the early stage of reperfusion. Notably, ferroptosis and necrosis emerged as the predominant forms of cell death during the medium-to-long-term reperfusion period.
Discussion:
In summary, this study demonstrates that apoptosis takes place during the early stage of reperfusion. Besides, ferroptosis, necrosis and pyroptosis played a crucial role in the prolonged I/R injury period.
Insights
This study reveals that apoptosis occurs early in myocardial ischemia-reperfusion injury. Ferroptosis and necrosis become dominant cell death modes during later reperfusion periods, impacting heart damage.
Area of Science:
- Cardiovascular Biology
- Cell Death Mechanisms
- Myocardial Infarction Research
Background:
- Reperfusion is crucial for myocardial infarction (MI) treatment but induces cardiac damage.
- Understanding cell death modes in ischemia-reperfusion (I/R) injury is vital, yet direct comparisons across reperfusion times are limited.
Purpose of the Study:
- To investigate the relationship between distinct cell death patterns and varying reperfusion durations.
- To compare the temporal dynamics of cell death categories in myocardial I/R injury.
Main Methods:
- Analysis of public sequencing data to identify cell death patterns.
- Evaluation of cell death timing in cellular and murine models across different reperfusion intervals (6h, 12h, 24h).
Main Results:
- Apoptosis is observed early in I/R injury and persists with increased reperfusion time.
- Autophagy and cuproptosis changes are prominent in the early reperfusion phase.
- Ferroptosis and necrosis become the primary cell death forms during medium-to-long-term reperfusion.
Conclusions:
- Apoptosis is an early event in reperfusion injury.
- Ferroptosis, necrosis, and pyroptosis are critical in prolonged myocardial I/R injury.
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